I'm not saying it's new or crazy. I'm saying it's inappropriate for DIY and the sweet spot for LPS between "adjuvent" and "lethal" is likely to be dangerously narrow. The margin for error with DIY-grade methods and equipment is probably far too large to permit it.
Not to mention that there are lots of other toll-activating goodies in dead bacteria, and people's severity of reaction to any given toll-activating antigen is probably strongly affected by genetics, so your dose response curve probably has variables outside your control or ability to factor.
So, again.. some things really belong in the pro-tier labs. Those labs should be more democratic, accountable, focused on public interest, and all.. but the basic idea that some experiments belong at that level of experience, resource, accountability, and group competence still seems sound to me.
This doesn't seem to be a new or crazy idea. A couple reviews:Bacterial ghosts as adjuvants: mechanisms and potential: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482964/
Bacterial Ghosts as antigen delivery vehicles: https://www.ncbi.nlm.nih.gov/pubmed/15878634/
On Friday, March 6, 2020 at 11:30:34 PM UTC-8, Cathal Garvey wrote:LPS is a bit too much for use as an adjuvent, I would think. And standardising the dose would be super important and super hard at DIY scale.
Generally I would say DIY is never injectable, and the skills and resources needed to do safe biomedicine are best acquired the traditional way.On 7 March 2020 04:32:31 GMT+00:00, Dakota Hamill <dko...@gmail.com> wrote:I'm no immunologist but I think that's how you'd give yourself toxic shock syndrome.On Fri, Mar 6, 2020 at 9:21 PM Michael Flynn <mfly...@gmail.com> wrote:Often in vaccine development, in addition to target antigens that you want to raise antibodies against, an adjuvant, something that stimulates the immune system like lipopolysaccharide, must be added in order to generate an adaptive immune response against the antigen. E.coli is covered with lipopolysaccharide, which reacts strongly to complement (antibacterial factors in blood), and so would be a good candidate for an adjuvant. This line of thinking leads to an "easy vaccine" where you express the antigen in E.coli and thermally lyse the cells, dilute them to some concentration in water for injection, and there you have your vaccine. In an outbreak like coronavirus, making vaccines like this would seem to be "low risk, high reward" in that nothing bad seems likely to come of it, but you could potentially immunize against this bad outbreak.--Anyone have any actual literature on this?
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diy...@googlegroups.com. To unsubscribe from this group, send email to diy...@googlegroups.com . For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diy...@googlegroups.com .
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/f04a71ff-46bf- .40b3-b5b2-600e7a5de47a% 40googlegroups.com
--
Sent from my Android device with K-9 Mail. Please excuse my brevity.
--
Sent from my Android device with K-9 Mail. Please excuse my brevity.
0 comments:
Post a Comment