Re: [DIYbio] Electroporator purchase help / advice sought

Hi folks,

Thanks for sharing your experience, expertise and even offering to send / sell me your
fabricated shocking chamber. Much appreciated.

I am a biologist, and not a real DIYer. Since I am so used to university
based labs, I bit the bullet and purchased a used Bio-Rad electroporation setup
including the gene pulser, pulse controller, capacitance extender and the shocking chamber.

This was a $550 purchase, which is not small change for garage lab operators like me. So
I hope it works as it is expected to.

But in case this set up fails to work as expected, I'll be sure to ping you helpful
folks again.

Pierre Padilla - The Portoporator is super cool and interesting. My friend did contact
Dr. Daniel Gilbert in Germany, who suggested getting local help for fabrication.
This same friend also contacted Dr. Saad Bhamla at Georgia Tech, of ElectroPen fame,
and he also suggested getting local help. So connecting with local DIY talent is something
I need to pursue actively...
A few, select ElectroPen / Saad Bhamla links -
https://twitter.com/bhamlalab/status/1076331619743608832?lang=eu
https://twitter.com/bhamlalab/status/1076334209923534849?lang=en
https://www.youtube.com/watch?v=yPwn0nexCtU
https://www.biorxiv.org/content/10.1101/448977v1

John Griessen - I don't think I have the skill set to use your custom shocking chamber.
But should the BioRad setup arrives soon not work as expected, I will take you up on your
offer.

Nathan McCorkle - Agreed, I need to establish connections with the local community college,
where I am sure undergrad kids have experience and expertise with additive manufacturing.
Thanks also for the warning about minimizing time between shock and medium addition.

Cheers,
Tom