Re: [DIYbio] DIY gene therapy for epigenome reset

DIY epigenetic gene therapy is, I'm sorry to say, a very bad idea and generally against the ethos of "medically supportable and safe activities only" around here.

To say nothing of the usual risks of insertable gene therapies themselves (though AAV is one of the less risky methods, sure), we really don't know enough about promoter/miRNA/inter-regulatory stuff in the real-world, high-variable-genetic-background, seriously-not-just-in-practically-clonal-mice environment of human bodies. It's a recipe for disaster to set off and try to DIY this stuff.

The least that could go wrong is septicaemia when one injects a homebrew formula. Or immune explosions due to trace antigens from your strains. Climbing the ladder, you get potential autoimmune responses to any successfully transfected cells, and you hope you don't get cross reactivity against other cells and self-antigens.

Just, uh, don't go there. Gene therapy csn be done..by top-tier professionals under tight supervision and sanity checking from their peers and medical/ethical overseers.

Instead, see what we can learn from the mice studies about endogenous regulation, and see what can be done without resorting to shotgun tools like gene therapy. The DIY hack might be learning that this effect could be stimulated to some extent by some OTC medicine with a good safety profile, or by a specific dietary intervention, etcetera. And there _is_ a lot of scope for that if you learn how to navigate expression/interaction databases and primary scientific literature. I would encourage you to investigate here instead.