PCR was originally done by setting up three water baths at different temperatures (annealing 55-65C, extension 72C, denaturation-boiling), and moving the PCR reaction tubes by hand between water baths. This is the easiest way to test PCR. Estimate about 2 min total per cycle, in one hour a PCR amplification can be done. This gets tedious--sitting there, watching a timer--which is why PCR machines were developed, but you can start trying it without building/buying a machine.
Cheers,
Jim Lund
Hello everyone,I am biology teacher and I discovered this DIYbio movement one week ago, and it is amaizing. I am thinking of buying a thermal cycler for me and maybe then bring it somehow to the school.I would like to know your opinion about the 2 opensource thermal cycler that I have found:I have read about this one and it seems it works well.This is much cheaper and for me would be perfect buy this one, but I know nothing about this one and it seems too simple...Futher, I would like to ask if you think it is a bit crazy and if it's going to be too much
expensive the rest of material, primers, buffers,... If I just want to play in my home.thanks,
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