I did most of my PhD thesis work using an OpenPCR, too bad they aren't selling it anymore. The Ninja actually looks pretty similar to the OpenPCR (maybe the same heatblock?). If you are close to a research university, I'd also recommend seeing if they have a used/recycled equipment store. Many universities do, and they will often have old lab equipment. I've picked up one or two old PCR machines at the University of Minnesota's ReUse store.
--Bryan Jones
On Mon, Aug 17, 2020 at 10:46 AM jlund256 <jlund256@gmail.com> wrote:
--PCR was originally done by setting up three water baths at different temperatures (annealing 55-65C, extension 72C, denaturation-boiling), and moving the PCR reaction tubes by hand between water baths. This is the easiest way to test PCR. Estimate about 2 min total per cycle, in one hour a PCR amplification can be done. This gets tedious--sitting there, watching a timer--which is why PCR machines were developed, but you can start trying it without building/buying a machine.Cheers,Jim LundOn Wednesday, August 12, 2020 at 3:33:22 PM UTC-5, NicVied wrote:Hello everyone,I am biology teacher and I discovered this DIYbio movement one week ago, and it is amaizing. I am thinking of buying a thermal cycler for me and maybe then bring it somehow to the school.I would like to know your opinion about the 2 opensource thermal cycler that I have found:I have read about this one and it seems it works well.This is much cheaper and for me would be perfect buy this one, but I know nothing about this one and it seems too simple...Futher, I would like to ask if you think it is a bit crazy and if it's going to be too much
expensive the rest of material, primers, buffers,... If I just want to play in my home.thanks,
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