Thanks a lot for all of your answers. Sorry that I wasn't more specific in my initial post. The 500 $ are only for the camera. Filters are already set up. I have to measure fluorescence of moving particles with at least 20 samples per second (20 fps). Furthermore the sensor should be applicable for a broader spectrum. Right now I only have to detect one fluorophore. Besides that I basically don't have any constraints.
The moving particles are in a tube with fixed wall thickness. These particles are only moving in one direction and have a constant speed. So it's fine that I only measure the fluorescence directly in front of my sensor. I have to detect their presence/absence. I am triggering the fluorescence by illuminating the particles with light from a LED. I want to place my sensor perpendicular to the light source.
The moving particles are in a tube with fixed wall thickness. These particles are only moving in one direction and have a constant speed. So it's fine that I only measure the fluorescence directly in front of my sensor. I have to detect their presence/absence. I am triggering the fluorescence by illuminating the particles with light from a LED. I want to place my sensor perpendicular to the light source.
John Griessen schrieb am Montag, 23. November 2020 um 20:08:06 UTC+1:
On 11/23/20 5:46 AM, 'Charles Maldonado' via DIYbio wrote:
> Hi all,
>
> I am developing a DIY detection sytem for fluorescence. I want to do the actual detection with a camera, but right now I am
> struggling a little bit with the selection of this camera There are so many cameras available and I don't know, which one of them
> will allow me to detect fluorescence. I guess, I need operation in low-light conditions and a high SNR. But aside from that, I
> don't know, which minimal specs I need...
> I just want to use it for detection, not for imaging.
Hi Charles,
I like Lisa's suggestion of a component very much if you are dealing with a zone of light. If you have a cell colony and want to
choose the brightest pixel out of a blob or growing cells, an image sensor (camera) would be good, otherwise if it makes a zone of
uniform light (maybe from a vial or cuvette) it can be lined up with the field of view of a sensor and get good repeatable results
when using a vial to hold "stuff" and get light out of one side of it. What kind of it are you dealing with?
Do you have a way to control where your fluorescing "thing/suspension/slide/dish" is in front of the camera/sensor? For instance
if you have a cell suspension in a particular vial with a certain wall thickness of glass you could calibrate that for a light
intensity and it wold work fine if the shape of it fits somewhere so it always aims a zone of light the same way at a sensor.
--
John Griessen
blog.kitmatic.com Albuquerque NM building lab gear for biologists
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