Re: [DIYbio] Camera for fluorescence detection system

On 11/23/20 5:46 AM, 'Charles Maldonado' via DIYbio wrote:
> Hi all,
>
> I am developing a DIY detection sytem for fluorescence. I want to do the actual detection with a camera, but right now I am
> struggling a little bit with the selection of this camera There are so many cameras available and I don't know, which one of them
> will allow me to detect fluorescence. I guess, I need operation in low-light conditions and a high SNR. But aside from that, I
> don't know, which minimal specs I need...
> I just want to use it for detection, not for imaging.

Hi Charles,

I like Lisa's suggestion of a component very much if you are dealing with a zone of light. If you have a cell colony and want to
choose the brightest pixel out of a blob or growing cells, an image sensor (camera) would be good, otherwise if it makes a zone of
uniform light (maybe from a vial or cuvette) it can be lined up with the field of view of a sensor and get good repeatable results
when using a vial to hold "stuff" and get light out of one side of it. What kind of it are you dealing with?

Do you have a way to control where your fluorescing "thing/suspension/slide/dish" is in front of the camera/sensor? For instance
if you have a cell suspension in a particular vial with a certain wall thickness of glass you could calibrate that for a light
intensity and it wold work fine if the shape of it fits somewhere so it always aims a zone of light the same way at a sensor.

--
John Griessen
blog.kitmatic.com Albuquerque NM building lab gear for biologists

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/362d4833-1b64-3655-b08c-c0ab655ca394%40industromatic.com.