Re: Re[4]: [DIYbio] How does one hire a bio lab technician for consulting?

Btw in my experience CVS is the worst about filling testosterone prescriptions . They will take a 10 mL prescription and tell you that they have to give it to u in 1cc allotments because it's a controlled substance . they also charge more than any other pharmacy for that and everything else. You might want to consider getting a prescription but not using insurance to pay for it because it's really not that expensive especially if you use a coupon from good RX and then they cannot do anything but give you the full prescription since you are paying 

On January 14, 2021 6:44:01 PM Dakota Hamill <dkotes@gmail.com> wrote:

I'd talk for days about hormones. That website is awesome, thanks
Frank.  I ended up finding a urologist via CVS who runs a men's clinic
on the side but I can't seem to get him to take over my insurance
covered TRT because...I can't even get an appointment with him.  It's
super frustrating being on TRT after  4 endos, and the one who finally
agreed to treat me doesn't believe in AI's or hCG.

I'm a keep this thread rolling with the hijack if that's ok Frank lol.
I don't know why there isn't a crowd-sourced diagnostic platform to
help people (not give medical advice) but give "ideas" about what to
test or what may be a root cause.

https://imgur.com/a/FpWVqUe

That was my development on TRT.  I basically went from 0 facial hair
and body hair my entire life to a beard in 10 months, I consider that
I'm finishing puberty at 33.  Psychologically it's fucked me.  Science
the only thing that kept me alive.

I was diagnosed with normosmic idiopathic hypogonadotropic
hypogonadism but I don't think that's the case.  I agree with
hypogonadism without a known cause...but I don't think it's Kallmann
syndrome at all.  I KNOW there is something wrong with my sympathetic
nervous system because I've been in fight-or-flight mode since 5 years
old, and nothing horrific happened.

I've been pouring sweat since 5/6 years old after a Chicken Pox
infection.  My hands are dripping liquid as I write this...and there's
no reason for that.

Always had thermal dysregulation, always felt hot, feet and hands cold
sweat.  Went from skinny as a rail as a track athlete to obese (with
that visceral fat), insulin resistant, metabolic syndrome,
dyslipidemia, heart issues, dermatographia, spider veins everywhere,
massive stretch marks.

I've just felt like I have been high on adrenaline/cortisol my entire
life, and never able to relax, and it took a toll.  But...my cortisol
always comes back normal, as did my catecholamines.

Sympathetic nervous system will shut down GI movement, and I also had
horrible GI bleeding and constipation, with joint aches (first
testosterone was 171ng/dL with a 7 total estradiol, both below range
and bottom of range).

TRT my GI bleeding went away within 48 hours after 5 years of bleeding
(never did a colonoscopy), joint pain went away (finally had
estrogen), lipids normalized, insulin normalized, blood sugar
normalized.

Only thing is I still have hyperhidrosis for no reason, and I feel
anxious all the time and had a few bouts of mania/psychosis on TRT.
Idk if my thyroid is fucked or my adrenals are fucked or I have a
tumor somewhere since 5 years old or what.

Well maybe someone smarter than me in this can help.  I've just felt
tired...forever.

On Thu, Jan 14, 2021 at 5:09 PM Frank <fgarcia0007@gmail.com> wrote:

To better answer the question that you actually asked, yes the testosterone that I've taken has always been intramuscular and by prescription although I have definitely taken my shot at getting testosterone without a prescription. I would not recommend it.

there is a way to discover what doctors in your area are likely to prescribe testosterone to you . The Medicare payment database is made available through an organization called propublica . heres a link to the resource with testosterone selected. https://projects.propublica.org/checkup/drugs/8883 just sort results by state and look up your state and you'll see the top testosterone prescribing doctors.

------ Original Message ------
From: "Tom De Medts" <tdmedts@gmail.com>
To: "DIYbio" <diybio@googlegroups.com>
Sent: 1/13/2021 3:23:43 PM
Subject: Re: Re[2]: [DIYbio] How does one hire a bio lab technician for consulting?

@ Dakota - Is your testosterone cypionate a prescription intra-muscular injection? And is it possible to procure this without a prescription?

I have stubborn abdominal visceral fat that just will not go away to the point that exercising with that objective has led to multiple ligaments tears of the knees,
at least 3 vertebral disc wear requiring future surgery, bad low-back with very limited flexibility, displacement of cervical vertebrae etc.

I've heard that testosterone injections generally help with healing better and faster, is this true?

Also, my testosterone levels were normal in my recent blood test, but I wonder whether its levels vary just as levels of most female hormones cycle up and down? Perhaps my androgen levels
seem OK in a snapshot, but not if followed as a time course?  Like some days, in the morning especially, my voice will be deeper. And other times, my  voice will sound less deep, a little more squeaky. As a non-medical biologist, I would not venture to hypothesize if these are all related or not.  So I'm just thinking aloud and throwing out these observations.

Any suggestions / advice is welcome...
@ Frank and Dakota, this is not at all an effort to hijack your discussion. Sorry if this is going off on a slight tangent, happy to start a new thread if you wish...

Thank you, in advance.

Cheers,
TdM


On Wed, Jan 13, 2021 at 1:09 PM Dakota Hamill <dkotes@gmail.com> wrote:

Seems like you have a solid reason.  Apologies for projecting the
notion of vanity on your decisions.   Outside of anti-aging clinics or
treating rare diseases I'm not sure who offers treatments like this
with stem cells.  How you separate who is legit from a quack, no idea
either.  I'm not even sure what FDA approved stem cell treatments
there are.

I can see the allure of anabolic steroids, I'm just on testosterone
cypionate now and it's reversed all sorts of problems I've had my
entire life overnight.

Steroids wouldn't help with your bone loss and teeth problems?
They're designed to help fight osteoporosis.

On Wed, Jan 13, 2021 at 12:09 AM Frank <fgarcia0007@gmail.com> wrote:

thanks for the message and for being concerned. I am familiar with body
dysmorphia more as an observer than a suffer. waaaaay back when I was
young I did my stint with body building and steroids. I'm lucky that it
did not turn into an addiction for me. I have friends who did not fare
so well. One of them , a radiologist with whom I attended medical school
died at the age of 31 from a massive heart attack resulting from
cardiomegaly.
Today I am the poster child for just about every damn age-related
degenerative condition, I had three artificial intervertebral disks in
my neck (before they were approved) due too herniated disks. The veins
in my legs are basically useless forcing me to wear compression socks
always. I got Halux Valgus (bunions) in both feet almost overnight, and
while i have no cavities,  I do have chronic aggressive periodontal
disease  with bone loss.  There's more but i'll hold off.   two of my
conditions have been shown to respond and even resolve with stem cell
therapy. With periodontitis, stem cell injections right on the receding
edge of the bone in a matrix that keeps them there for a little while
significantly stimulates not just bone but all the lost tissues to grow.
That treatment is actually in practice but it costs a fortune and I'm
about to loose 4 teeth if i don't act soon,.
the other which i have mentioned is more of a problem with even less of
a solution but in which stem cells have shown remarkable efficacy..
Believe me i have no thing about wanting to inject stem cells for
vanity. I would love to not have to go through all this trouble, So no
danger of me doing this just for th hell of it.

To your other point, I am absolutely into anti aging but in a true way,
not just superficially. If i had the money i would have a protocol for
myself that attacks some of the key drives and pay people to  force me
to do things like intermittent fasting, being in bed by midnight, and
exercising consistently. that right there is has more impact than just
about any single drug. Then I'd buy myself a plasmapheresis machine, and
get my hands on  4 or 5 of the molecules in this list:
https://docs.google.com/document/d/1c79BsB8ooKkefmKLNRwTj9F1grkyrEN0_vwwuR3gFmA/edit?usp=sharing

so my metric for "youthfulness" would not be things like wrinkles but
rather LDL/HDL ratio, GFR, C-reactive protein, HBAC, blood pressure,
physical strength, cognitive performance,  etc.




------ Original Message ------
From: "Dakota Hamill" <dkotes@gmail.com>
To: "diybio@googlegroups.com" <diybio@googlegroups.com>
Sent: 1/12/2021 9:45:09 PM
Subject: Re: [DIYbio] How does one hire a bio lab technician for
consulting?

I think you have an interesting project idea from your previous posts
and literature research, but it seems like you'll run into a number of
issues.  I hesitate to ask the "why" of why you want to re-inject
adipocyte stem-cells back into yourself because usually people that
want to do something like this have reasons no one else can talk them
out of.

Not judging though, because I have some serious pathological body
dysmorphia issues from a rare endocrine disorder, and I'm on
endocrinologist supervised treatment, but it never feels like enough.
I assume this is what it feels like to be anorexic or obsessed with
plastic surgery.  People see things about themselves no one else does.

If you're dead set on it, you're probably going to have to do it
yourself.  That's going to make it difficult and expensive, and
dangerous.  You could die from an infection or immune response.  I
assume everyone has a "right" to self-treat themselves, but to what
extent another may be liable for manslaughter if it goes awry, I have
no idea.

What's your end measurable of it "working"?  Never aging?  No
wrinkles? No cellulite? You're going to inject it all over your entire
body that's covered with fat tissue?

Skip the headache/lawsuit/hospital bill of doing it yourself if you're
dead-set on it and talk to
https://www.bostonstemcell.com/stem-cell-therapy/fat-graft/  at least
you can sue a doctor if it goes awry.  $900-$6,000 and only one google
away!

Good luck hope you find what you're looking for.

On Tue, Jan 12, 2021 at 4:37 PM Frank <fgarcia0007@gmail.com> wrote:



As you know I am attempting to produce an sample of viable fat stem cells suitable for personal medical use, on my own, and on a budget. At this point I think I understand the salient biological and medical issues but I am lacking hands on laboratory know-how.
I looked into utilizing a community biospace but they are nonexistent close by or not interested in what I'm doing. Renting space in a biolab is too expensive.
So I was thinking that a way to accomplish this might be to hire as a consultant an experienced lab technician who has done all of the parts of what I'm attempting to do.
they would evaluate the protocol I'm gonna follow , tell me what to expect and about pitfalls, make sure i know all needed materials and equipment, and walk me through it. They could also tell me were I can use cheaper equipment alternatives .

So my question to this group: are biolab tech folks hired for this sort of thing? Does someone doing stem cell culturing at a biotech company ever moonlight to make extra money? If so is there a preferred source for such a consultant like a forum or classified?

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