Re: Re[4]: [DIYbio] How does one hire a bio lab technician for consulting?

I'd talk for days about hormones. That website is awesome, thanks
Frank. I ended up finding a urologist via CVS who runs a men's clinic
on the side but I can't seem to get him to take over my insurance
covered TRT because...I can't even get an appointment with him. It's
super frustrating being on TRT after 4 endos, and the one who finally
agreed to treat me doesn't believe in AI's or hCG.

I'm a keep this thread rolling with the hijack if that's ok Frank lol.
I don't know why there isn't a crowd-sourced diagnostic platform to
help people (not give medical advice) but give "ideas" about what to
test or what may be a root cause.

https://imgur.com/a/FpWVqUe

That was my development on TRT. I basically went from 0 facial hair
and body hair my entire life to a beard in 10 months, I consider that
I'm finishing puberty at 33. Psychologically it's fucked me. Science
the only thing that kept me alive.

I was diagnosed with normosmic idiopathic hypogonadotropic
hypogonadism but I don't think that's the case. I agree with
hypogonadism without a known cause...but I don't think it's Kallmann
syndrome at all. I KNOW there is something wrong with my sympathetic
nervous system because I've been in fight-or-flight mode since 5 years
old, and nothing horrific happened.

I've been pouring sweat since 5/6 years old after a Chicken Pox
infection. My hands are dripping liquid as I write this...and there's
no reason for that.

Always had thermal dysregulation, always felt hot, feet and hands cold
sweat. Went from skinny as a rail as a track athlete to obese (with
that visceral fat), insulin resistant, metabolic syndrome,
dyslipidemia, heart issues, dermatographia, spider veins everywhere,
massive stretch marks.

I've just felt like I have been high on adrenaline/cortisol my entire
life, and never able to relax, and it took a toll. But...my cortisol
always comes back normal, as did my catecholamines.

Sympathetic nervous system will shut down GI movement, and I also had
horrible GI bleeding and constipation, with joint aches (first
testosterone was 171ng/dL with a 7 total estradiol, both below range
and bottom of range).

TRT my GI bleeding went away within 48 hours after 5 years of bleeding
(never did a colonoscopy), joint pain went away (finally had
estrogen), lipids normalized, insulin normalized, blood sugar
normalized.

Only thing is I still have hyperhidrosis for no reason, and I feel
anxious all the time and had a few bouts of mania/psychosis on TRT.
Idk if my thyroid is fucked or my adrenals are fucked or I have a
tumor somewhere since 5 years old or what.

Well maybe someone smarter than me in this can help. I've just felt
tired...forever.

On Thu, Jan 14, 2021 at 5:09 PM Frank <fgarcia0007@gmail.com> wrote:
>
> To better answer the question that you actually asked, yes the testosterone that I've taken has always been intramuscular and by prescription although I have definitely taken my shot at getting testosterone without a prescription. I would not recommend it.
>
> there is a way to discover what doctors in your area are likely to prescribe testosterone to you . The Medicare payment database is made available through an organization called propublica . heres a link to the resource with testosterone selected. https://projects.propublica.org/checkup/drugs/8883 just sort results by state and look up your state and you'll see the top testosterone prescribing doctors.
>
> ------ Original Message ------
> From: "Tom De Medts" <tdmedts@gmail.com>
> To: "DIYbio" <diybio@googlegroups.com>
> Sent: 1/13/2021 3:23:43 PM
> Subject: Re: Re[2]: [DIYbio] How does one hire a bio lab technician for consulting?
>
> @ Dakota - Is your testosterone cypionate a prescription intra-muscular injection? And is it possible to procure this without a prescription?
>
> I have stubborn abdominal visceral fat that just will not go away to the point that exercising with that objective has led to multiple ligaments tears of the knees,
> at least 3 vertebral disc wear requiring future surgery, bad low-back with very limited flexibility, displacement of cervical vertebrae etc.
>
> I've heard that testosterone injections generally help with healing better and faster, is this true?
>
> Also, my testosterone levels were normal in my recent blood test, but I wonder whether its levels vary just as levels of most female hormones cycle up and down? Perhaps my androgen levels
> seem OK in a snapshot, but not if followed as a time course? Like some days, in the morning especially, my voice will be deeper. And other times, my voice will sound less deep, a little more squeaky. As a non-medical biologist, I would not venture to hypothesize if these are all related or not. So I'm just thinking aloud and throwing out these observations.
>
> Any suggestions / advice is welcome...
> @ Frank and Dakota, this is not at all an effort to hijack your discussion. Sorry if this is going off on a slight tangent, happy to start a new thread if you wish...
>
> Thank you, in advance.
>
> Cheers,
> TdM
>
>
> On Wed, Jan 13, 2021 at 1:09 PM Dakota Hamill <dkotes@gmail.com> wrote:
>>
>> Seems like you have a solid reason. Apologies for projecting the
>> notion of vanity on your decisions. Outside of anti-aging clinics or
>> treating rare diseases I'm not sure who offers treatments like this
>> with stem cells. How you separate who is legit from a quack, no idea
>> either. I'm not even sure what FDA approved stem cell treatments
>> there are.
>>
>> I can see the allure of anabolic steroids, I'm just on testosterone
>> cypionate now and it's reversed all sorts of problems I've had my
>> entire life overnight.
>>
>> Steroids wouldn't help with your bone loss and teeth problems?
>> They're designed to help fight osteoporosis.
>>
>> On Wed, Jan 13, 2021 at 12:09 AM Frank <fgarcia0007@gmail.com> wrote:
>> >
>> > thanks for the message and for being concerned. I am familiar with body
>> > dysmorphia more as an observer than a suffer. waaaaay back when I was
>> > young I did my stint with body building and steroids. I'm lucky that it
>> > did not turn into an addiction for me. I have friends who did not fare
>> > so well. One of them , a radiologist with whom I attended medical school
>> > died at the age of 31 from a massive heart attack resulting from
>> > cardiomegaly.
>> > Today I am the poster child for just about every damn age-related
>> > degenerative condition, I had three artificial intervertebral disks in
>> > my neck (before they were approved) due too herniated disks. The veins
>> > in my legs are basically useless forcing me to wear compression socks
>> > always. I got Halux Valgus (bunions) in both feet almost overnight, and
>> > while i have no cavities, I do have chronic aggressive periodontal
>> > disease with bone loss. There's more but i'll hold off. two of my
>> > conditions have been shown to respond and even resolve with stem cell
>> > therapy. With periodontitis, stem cell injections right on the receding
>> > edge of the bone in a matrix that keeps them there for a little while
>> > significantly stimulates not just bone but all the lost tissues to grow.
>> > That treatment is actually in practice but it costs a fortune and I'm
>> > about to loose 4 teeth if i don't act soon,.
>> > the other which i have mentioned is more of a problem with even less of
>> > a solution but in which stem cells have shown remarkable efficacy..
>> > Believe me i have no thing about wanting to inject stem cells for
>> > vanity. I would love to not have to go through all this trouble, So no
>> > danger of me doing this just for th hell of it.
>> >
>> > To your other point, I am absolutely into anti aging but in a true way,
>> > not just superficially. If i had the money i would have a protocol for
>> > myself that attacks some of the key drives and pay people to force me
>> > to do things like intermittent fasting, being in bed by midnight, and
>> > exercising consistently. that right there is has more impact than just
>> > about any single drug. Then I'd buy myself a plasmapheresis machine, and
>> > get my hands on 4 or 5 of the molecules in this list:
>> > https://docs.google.com/document/d/1c79BsB8ooKkefmKLNRwTj9F1grkyrEN0_vwwuR3gFmA/edit?usp=sharing
>> >
>> > so my metric for "youthfulness" would not be things like wrinkles but
>> > rather LDL/HDL ratio, GFR, C-reactive protein, HBAC, blood pressure,
>> > physical strength, cognitive performance, etc.
>> >
>> >
>> >
>> >
>> > ------ Original Message ------
>> > From: "Dakota Hamill" <dkotes@gmail.com>
>> > To: "diybio@googlegroups.com" <diybio@googlegroups.com>
>> > Sent: 1/12/2021 9:45:09 PM
>> > Subject: Re: [DIYbio] How does one hire a bio lab technician for
>> > consulting?
>> >
>> > >I think you have an interesting project idea from your previous posts
>> > >and literature research, but it seems like you'll run into a number of
>> > >issues. I hesitate to ask the "why" of why you want to re-inject
>> > >adipocyte stem-cells back into yourself because usually people that
>> > >want to do something like this have reasons no one else can talk them
>> > >out of.
>> > >
>> > >Not judging though, because I have some serious pathological body
>> > >dysmorphia issues from a rare endocrine disorder, and I'm on
>> > >endocrinologist supervised treatment, but it never feels like enough.
>> > >I assume this is what it feels like to be anorexic or obsessed with
>> > >plastic surgery. People see things about themselves no one else does.
>> > >
>> > >If you're dead set on it, you're probably going to have to do it
>> > >yourself. That's going to make it difficult and expensive, and
>> > >dangerous. You could die from an infection or immune response. I
>> > >assume everyone has a "right" to self-treat themselves, but to what
>> > >extent another may be liable for manslaughter if it goes awry, I have
>> > >no idea.
>> > >
>> > >What's your end measurable of it "working"? Never aging? No
>> > >wrinkles? No cellulite? You're going to inject it all over your entire
>> > >body that's covered with fat tissue?
>> > >
>> > >Skip the headache/lawsuit/hospital bill of doing it yourself if you're
>> > >dead-set on it and talk to
>> > >https://www.bostonstemcell.com/stem-cell-therapy/fat-graft/ at least
>> > >you can sue a doctor if it goes awry. $900-$6,000 and only one google
>> > >away!
>> > >
>> > >Good luck hope you find what you're looking for.
>> > >
>> > >On Tue, Jan 12, 2021 at 4:37 PM Frank <fgarcia0007@gmail.com> wrote:
>> > >>
>> > >>
>> > >>
>> > >> As you know I am attempting to produce an sample of viable fat stem cells suitable for personal medical use, on my own, and on a budget. At this point I think I understand the salient biological and medical issues but I am lacking hands on laboratory know-how.
>> > >> I looked into utilizing a community biospace but they are nonexistent close by or not interested in what I'm doing. Renting space in a biolab is too expensive.
>> > >> So I was thinking that a way to accomplish this might be to hire as a consultant an experienced lab technician who has done all of the parts of what I'm attempting to do.
>> > >> they would evaluate the protocol I'm gonna follow , tell me what to expect and about pitfalls, make sure i know all needed materials and equipment, and walk me through it. They could also tell me were I can use cheaper equipment alternatives .
>> > >>
>> > >> So my question to this group: are biolab tech folks hired for this sort of thing? Does someone doing stem cell culturing at a biotech company ever moonlight to make extra money? If so is there a preferred source for such a consultant like a forum or classified?
>> > >>
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