very good points and questions which i would certainly be on the agenda in a wider experiment that had some resources. I am attempting simply to carry out a procedure that within narrow parameters of safety which most experts agree should be relatively safe and also efficacious but which, as far I know hasn't yet been put into practice. What makes this compelling for me is the combination of 5 factors:
- Immediate need to slow or halt periodontal disease
- High potential for significant improvement in periodontal disease progression
- High safety profile - if basic rules are followed it is pretty safe
- Only one or two treatments needed
- Very low cost
I'm assuming periodontal professionals would be interested to see how that works out. They should be able to provide the details needed to design an adequate protocol. Those details would include
- Donor selection criteria (oral characteristics and features, etc.)
- Plaque harvest sites
- Plaque harvest technique
- criteria for vehicle for the plaque such as gel
- Recipient preparation
- Implant/placement method
- Post-transplant steps
If you can recommend a good way to find dental profession folks that would provide this type of feedback I'd appreciate it.
Here's an exerpt from a paper on the subject that proposes a protocol:
"Pozhitkov and collaborators proposed to introduce health-associated oral microbiota into the oral cavity of periodontitis patients24. First, they confirmed that the microbiome of subjects with periodontitis were distinct from those of healthy or edentulous patients. Next, they tested an in vitro antimicrobial protocol to be used on the oral cavity of the recipient patient prior to OMT. It was shown that application of sodium hypochlorite (NaOCl) followed by its neutralization with sodium ascorbate buffer may be a valid option for suppressing the disease-associate microbiota to allow for a more pronounced microbial shift to a healthier microbiota. In that same study, the authors suggested an OMT procedure consisting of: (1) collecting sub- and supra-gingival plaque from a healthy donor (spouse or a partner), (2) performing deep cleaning, root planning and applying a broad-spectrum antimicrobial agent to the periodontitis patient, and finally (3) neutralizing the antimicrobial agent immediately following by a rinsing with a microbial suspension harvested from the healthy donor in the periodontitis patient24."
Frank
------ Original Message ------
From: "Tom De Medts" <tdmedts@gmail.com>
To: "DIYbio" <diybio@googlegroups.com>
Sent: 3/21/2021 3:38:16 PM
Subject: Re: Re[2]: [DIYbio] Re: Oral microbiota transplant
Hi Frank,Seems like you've given this topic quite some background research. Bravo! :)Just curious if you've accounted for differential virome contributions to observed differences in dental healthFor example, see this article - https://naturemicrobiologycommunity.nature.com/posts/61634-the-oral-virome-a-new-driver-in-periodontal-disease-and-periodontal-arthritisThis also makes me wonder if you will, and how you will screen donors for viral infection so as to prevent oral inoculation with an un-verified donor (sample)- self-reported, or tested?Cheers!--On Fri, Mar 19, 2021 at 5:36 AM Frank <fgarcia0007@gmail.com> wrote:--To answer some of your questions.1. the jury is actually no longer out on the cause of PD. we know that gingivitis can be stable which means a healthy oral microbiome remains intact or it can progress to periodontal disease. This begins with an inflammatory response to gingivsl bacteria. but rather than end the inflammation as usual , the immune system which normally secretes lipid pro-resolving mediators, SPMs, such as resolvins, maresins, and lipoxins, fails to do so and the inflammation becomes chronic. inflammation destroys tissue. the persistent Inflammation and pocket formation changes the bacterial growth environment that favors the emergence of 'periodontal pathogens' and alters the diversity and relative proportions of species leading to a a dysbiotic microflora. the dysbiotic subgingival plaque microbiome drives further inflammation which destroys tissue and perpetuates a dysbiotic milieu. Mutually reinforcement between unresolving inflammation and dysbiotic microflora perpetuates the disease.it has now been shown that treating someone with PD with topical resolvins end the disease and results in bone regeneration. But topical SPMs are not available. you can, however, increase their synthesis by taking high dose omega-3. combining that with an external correction of the oral microbiota could be enough to tip the balance enough to significantly resolve the disease.unlike many other experiments testing treatments, this is extremely safe. i can't think of any dangers.As far as NGS, l am unfortunately not a millionaire so that's not happening. it's also not necessary. the purpose of this experiment is to see if i notice a marked improvement in my PD. I just had xrays and a periodontal evaluation . if i do this I would return to the dentist for and reevaluation. after a few months.Best,Frank------ Original Message ------From: "Tom De Medts" <tdmedts@gmail.com>To: "DIYbio" <diybio@googlegroups.com>Sent: 3/19/2021 1:59:29 AMSubject: Re: [DIYbio] Re: Oral microbiota transplantratherFrank,A quick Google search for 'oral microbiota transplant' yielded at least 2 relevant hits:My suggestion would be to figure out what has been done already, summarize it as would at a lab meeting, in 3-5 slides,then figure out what has NOT been done yet, and proceed from there.It's quite possible you've completed all of those steps already, in which case, I'd be curious to read your summary oncurrent state of the art and what you intend to do specifically...i am assuming you'd want to perform NGS analyses of thehealthy donorrecipient pre-transplantrecipient post-transplant (how many time points)as you pointed out, from what specific dental location would the donor sample be obtained from?as you also pointed out, to what specific dental locations would the donor samples be transplanted to?So many questions.Initially, when I read your brief one liner intro email, I was alarmed because I thought it's been established thatdental plaque is the same type of plaque that is responsible for arterial disease etc.Then I realized 2 mistakes in my quick reading:1. You are planning to transplant from healthy donor to diseased (PD) individual, NOT the other way around.2. The jury is still out on whether plaque from PD is caused or simply correlated or not even that with respect to arterial plaque.I am neither a dentist nor a medical doctor, but as a scientist I'd be very curious to know more about how your background researchhas molded your research hypothesis and protocol(s) thus far. Good luck!- Tom--On Wed, Mar 17, 2021 at 6:13 PM Frank <fgarcia0007@gmail.com> wrote:--thanks! so what if you're not a dentist , some of this is common sense or basic science so any feedback is welcome------ Original Message ------From: "Inigo Howlett" <inigowalkerhowlett@gmail.com>To: "DIYbio" <diybio@googlegroups.com>Sent: 3/16/2021 10:28:18 PMSubject: [DIYbio] Re: Oral microbiota transplant
cool idea, sadly I'm not a dentist--On Thursday, March 11, 2021 at 5:55:26 PM UTC-5 Frank wrote:Any dentists, periodontists, or dental school students out there willing to participate in a safe and exciting experiment? It involves taking a small amount of dental plaque from a healthy donor who is relatively free of caries and shows no signs of periodontal disease, then putting that plaque into the mouth of a recipient (who does suffer from PD). This would be done with the purpose of having the donor plaque bacteria colonize the recipient's mouth.please let me know
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