Re: Re[2]: [DIYbio] Re: Oral microbiota transplant

To answer some of your  questions.

1. the jury is actually no longer out on the cause of PD. we know that gingivitis can be stable which means a healthy oral microbiome remains intact or it can progress to periodontal disease.  This begins with an inflammatory response to gingivsl bacteria.  but rather than end the inflammation as usual , the immune system which normally secretes lipid pro-resolving mediators, SPMs, such as resolvins, maresins, and lipoxins,  fails to do so and the inflammation becomes chronic.  inflammation destroys tissue. the  persistent Inflammation and pocket formation changes the bacterial growth environment that favors the emergence of 'periodontal pathogens' and alters the diversity and relative proportions of species leading to a a dysbiotic microflora. the dysbiotic subgingival plaque microbiome drives further inflammation which destroys tissue and perpetuates a dysbiotic milieu. Mutually reinforcement between unresolving inflammation and dysbiotic microflora perpetuates the disease.

it has now been shown that treating someone with PD with topical resolvins end the disease and results in bone regeneration.  But topical SPMs are not available.  you can, however, increase their synthesis by taking high dose omega-3.  combining that with an external  correction of the oral microbiota  could be enough to tip the  balance enough to significantly resolve the disease.

unlike many other experiments testing treatments, this is extremely safe. i can't think of  any dangers.  

As far as NGS, l am unfortunately not a millionaire so that's not happening. it's also not necessary.  the purpose of this experiment is to see if i notice a marked improvement in my PD.  I just had xrays and a periodontal evaluation . if i do this I would return to the dentist for and reevaluation. after a few months.

https://www.ncbi.nlm.nih.gov/sites/myncbi/1BWHziisucnQs/collections/60529863/public/

https://www.ncbi.nlm.nih.gov/sites/myncbi/1BWHziisucnQs/collections/60529852/public/

Best,

Frank

------ Original Message ------

From: "Tom De Medts" <tdmedts@gmail.com>

To: "DIYbio" <diybio@googlegroups.com>

Sent: 3/19/2021 1:59:29 AM

Subject: Re: [DIYbio] Re: Oral microbiota transplant

rather

Frank,

A quick Google search for 'oral microbiota transplant' yielded at least 2 relevant hits:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5828680/

https://bmcoralhealth.biomedcentral.com/articles/10.1186/s12903-015-0109-4

My suggestion would be to figure out what has been done already, summarize it as would at a lab meeting, in 3-5 slides,

then figure out what has NOT been done yet, and proceed from there.

It's quite possible you've completed all of those steps already, in which case, I'd be curious to read your summary on

current state of the art and what you intend to do specifically...

i am assuming you'd want to perform NGS analyses of the 

healthy donor

recipient pre-transplant

recipient post-transplant (how many time points)

as you pointed out, from what specific dental location would the donor sample be obtained from?

as you also pointed out, to what specific dental locations would the donor samples be transplanted to?

So many questions.

Initially, when I read your brief one liner intro email, I was alarmed because I thought it's been established that

dental plaque is the same type of plaque that is responsible for arterial disease etc.

Then I realized 2 mistakes in my quick reading:

1. You are planning to transplant from healthy donor to diseased (PD) individual, NOT the other way around.

2. The jury is still out on whether plaque from PD is caused or simply correlated or not even that with respect to arterial plaque.

I am neither a dentist nor a medical doctor, but as a scientist I'd be very curious to know more about how your background research 

has molded your research hypothesis and protocol(s) thus far. Good luck!

- Tom

On Wed, Mar 17, 2021 at 6:13 PM Frank <fgarcia0007@gmail.com> wrote:

thanks! so what if you're not a dentist , some of this is common sense or basic science so any feedback is welcome

------ Original Message ------

From: "Inigo Howlett" <inigowalkerhowlett@gmail.com>

To: "DIYbio" <diybio@googlegroups.com>

Sent: 3/16/2021 10:28:18 PM

Subject: [DIYbio] Re: Oral microbiota transplant

cool idea, sadly I'm not a dentist

On Thursday, March 11, 2021 at 5:55:26 PM UTC-5 Frank wrote:

Any dentists, periodontists, or dental school students out there willing to participate in a safe and exciting experiment? It involves taking  a small amount of dental plaque from a healthy donor who is relatively free of caries and shows no signs of periodontal disease, then putting that plaque into the mouth of a recipient (who does suffer from PD). This would be done with the purpose of having the donor plaque bacteria colonize the recipient's mouth.

please let me know

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to a topic in the Google Groups "DIYbio" group.
To unsubscribe from this topic, visit https://groups.google.com/d/topic/diybio/1L-f2oA_Eeg/unsubscribe.
To unsubscribe from this group and all its topics, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/caefdcdb-cf17-45af-8630-8c04e9ec124dn%40googlegroups.com.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/em0f23f129-9584-4e96-9151-fb120ddffe70%40cabron.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/CANpz%3DfG5efOg6HFvH%3Dt%3D_R8sFs8d9VAzG7c9LiYwNnj6h%3DcNQQ%40mail.gmail.com.

--
-- You received this message because you are subscribed to the Google Groups DIYbio group. To post to this group, send email to diybio@googlegroups.com. To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com. For more options, visit this group at https://groups.google.com/d/forum/diybio?hl=en
Learn more at www.diybio.org
---
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To unsubscribe from this group and stop receiving emails from it, send an email to diybio+unsubscribe@googlegroups.com.
To view this discussion on the web visit https://groups.google.com/d/msgid/diybio/em940df7dd-c901-4b74-86a0-675453f4ff9d%40cabron.