On 01/31/2015 08:08 AM, Sunil Phani wrote:
> nothing to get alarmed about mammalian cell culture until it's being used up on human beings ...
It's just that "it" could be used on YOU accidentally as you do lab technique...
and then consider side effects of disease spread risk from your lab cultures, etc.
So, "study up" is all they are saying.
I don't think anyone was alarmed. But...
They care about you even though they don't know you.
They worry when you say non-chalantly, "nothing to get alarmed about mammalian cell culture"...
They worry comparing your non-spell checked emails to all the long sequences of exacting detail
needed to do mammalian cell culture, and worry that "It might not work out..." as Bob Allen,
a good horse trainer says fairly often to his students...
So, "study up", OK?
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Re: [DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII
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[DIYbio] Re: help me setup DIY-BIO home Lab to produce Recombinant Factor VIII
hello friends,i respect your gathered concerns and there is nothing to get alarmed about mammalian cell culture until it's being used up on human beings ...
and i thoroughly understand the intention behind cautioning me.. i am actually feeling very encouraged and will be continue to pursue my passion ....
now i am actually looking into adaptable approaches to produce Recombinant Factor VIII using pichia pastoris is a species of methylotrophic yeast.
Pichia is widely used for protein expression using recombinant DNA techniques.
i sincerely believe that you ppl and Diybio will continuously advise me in this regard and guide me to accomplish my goal rather than plotting the scopes of Diybio
"If your actions inspire others to dream more, learn more, do more and become more, you are a leader...."
and in my view Diybio is a leader and savant ppl like you made this group a leader in all aspects ...
friends pls through some light on pichia pastoris and its pros and cons
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