Science

Urgent requirement

Please send resume @ bipin@tresourceinc.com 

Hi,

I have an urgent requirement with one of my clients, details given below. If you find yourself suitable for the position, please send me your latest updated resume along with contact details. Please include your employer details as well.

Skills (ADVANCED/EXPERT):

extensively worked on AR and GL especially experience in Auto lock box and credit card solution implementation

Should have worked on GL reconciliations

Description:

Oracle ERP Finance Functional Consultant

a.Should have ~ 8 years of experience as finance functional consultant

b.Should have worked on at least 4 - R12 implementation project as solution architect

c.Should have extensively worked on AR and GL especially experience in Auto lock box and credit card solution implementation

d. Should have worked on GL reconciliations

Thanks & Regards

BIPIN RAJ

Sr. US IT Recruiter (Talent Acquisition)

Technology Resource Group Inc

3736 Hills-Dale Court,
Santa Clara, CA 95051

Direct: 408-709-1760 Ext : 818,

G talk: bipin.rgtalent@gmail.com

bipin@tresourceinc.com

                                              www.tech-resource.com

Hello everyone,

First time poster, longtime reader. Undergrad biochem major looking to learn more about mycology, microbiology, and synthetic and molecular biology.

I need to obtain DNA from some unknown endophytic fungi that I've been growing on both potato dextrose agar and in potato dextrose broth. My plan is to amplify the DNA I find via PCR and have it sequenced. Some protocols I've obtained from previous research call for a simple method that, in summary, involves crushing, sonicating, and heating fresh mycelia in water or TE buffer. This has been mostly unsuccessful for my colleagues, and I'd prefer to try a more thorough method. However, there seems to be a multitude of methods out there so I'd like to get some opinions.

I found an experiment similar to mine that used the Qiagen DNeasy Plant Mini Kit (http://aem.asm.org/content/77/17/6076), but the smallest set would cost me over two hundred dollars and I simply can't afford it.

A relatively simple method that stuck out to me was described here: http://link.springer.com/article/10.1007%2Fs10327-006-0300-1 , and a link to a PDF is here: https://www.dropbox.com/s/iiae8pg7lsmunle/Simple_Method_for_Fungal_DNA_Extraction_%28sarkosyl%29.pdf?dl=0 .

This procedure involves immersing mycelia in a buffer of Tris/EDTA/NaCl/sarkosyl, centrifugation, and ethanol precipitation. I have access to all components except for sarkosyl, a detergent that is apparently weaker than SDS and does not precipitate at cold temperatures. I am wondering if I can substitute SDS for sarkosyl and centrifuge at room temperature rather than 4 degC.

I'd like to know what people think of this method, or if there are any suggestions for other simple procedures. I am a biochemistry undergrad student and very, very new to fungi, but I'm eager to learn. Does anyone here know of anything I could try, or places I should look?

Thanks and have a good evening.

-Brendan