Looking for Senior JAVA Developer with DevOps , Sunnyvale, CA

7:11 AM |

Hi,

 

Please find the below position and respond back to me at praveen@sritechsolutions.com

 

Senior JAVA Developer with DevOps  (70-80% Java Developer & 20-30% DevOps Engineer)

Sunnyvale, CA

Contract Role


Please send me candidates if they have all experience what client needed....

 

Required Skills:

  • Candidate must have a development background in Java and expertise in Maven
  • Develop and Maintain DevOps tools using Java/J2EE
  • Programming Languages: Java
  • Binary Repository Manager - Nexus/Artifactory
  • Build system - Maven, Ant,
  • OS - RHEL, Centos
  • Scripting: BASH, Python, Perl
  • Config Mgmt: Ansible, Puppet, Chef
  • Prototyping: Vagrant, VMWare Workstation/Fusion
  • Front End Development using JavaScript, AngularJS etc. (NICE TO HAVE)

 

Thanks and Best Regards,

 

Praveen Kumar

SRI Tech Solutions Inc.

praveen@sritechsolutions.com

(T) 1-813-423-6500 X Ext: 149 (M) 813-440-2778 (F) 813.423.6520

 

Certified Minority Business Enterprise | An E-Verify Employer

SRI Tech Solutions is an equal opportunity employer and does not discriminate on the basis of race, color, gender, religion, age, sexual orientation, national origin or citizenship status or ethnic origin, disability, marital status, veteran status, or any other occupationally irrelevant criteria.

 

 

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Posted by Science

[DIYbio] Re: Eating Glow in the dark yogurt

6:36 AM |

I PCRed one of the native CRISPRs out, believe it had AGAAW or something like that as the PAM. I didn't have enough funding or time to fully clone it, but I do plan on it soon. If I remember correctly, the crRNA is on the 3' of the Cas9 rather than the 5' like normal Cas9. Would be convenient if you need hard regulation of it's expression.  

https://www.amazon.com/gp/product/B001MV5PB0/ref=oh_aui_search_detailpage?ie=UTF8&psc=1

PCRed a 500bp fragment of that Cas9 off of this. It was a couple years ago though. There's like 2-3 CRISPRs in it I think, depending on what strain you have. 


I also think I have a broad range gram positive plasmid that expressed GFP from B subtilis. Would have to dig that up. Would most likely work in S thermo. I think the yogurt starter has one other strain though. 

-Koeng

On Tuesday, May 31, 2016 at 5:38:11 AM UTC, Patrik D'haeseleer wrote:
Here's an interesting thought I've been toying with:

One of the classical yogurt making bacteria is Streptococcus thermophilus, a close relative of S. pyogenes, which is the source of the canonical Cas9 gene.

Turns out that S. thermophilus has a native Cas9 system. So in theory, all we need to do genome editing in S. thermophilus would be to provide it with an artificial CRISPR casette!

Completely bypasses any IP issues around Cas9 as well...  :-)

Patrik

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