Re: [DIYbio] Re: Were the DIY CRISPR kits contaminated?

From the Germans (science bridge): still the same. Got  new bacteria from Don Court, we get StrepR but no correct editing. Our post on Odiham Page is not released. Zayner does not respond to mail.


On 15 Jan 2018 03:15, "August Pamplona" <cosmicaug@gmail.com> wrote:
On Tuesday, December 26, 2017 at 9:01:47 AM UTC-5, Wolfgang Nellen wrote:

Did you get any response from the Germans yet?
Concerning communication: is there anything like a Facebook site where all communities could talk to each other and discuss issues like the kit? I guess the authorities do not have a clue where the DIY communities are!


Hi,

        There are some. Try https://www.facebook.com/groups/diybio/ and https://www.facebook.com/groups/769326966539324/ . This might be relevant too: https://www.facebook.com/groups/biohackacademy/ 

        Also, if you are into fungi these might be sort of relevant: https://www.facebook.com/groups/FungalSequencing/https://www.facebook.com/groups/fungalmaterials/

        There are many other possible matches.

August


 
On 22 Dec 2017 20:00, "Vanessa lorenzo toquero" <vloren...@gmail.com> wrote:
Hi,

I introduce myself. Vanessa Lorenzo from Hackuarium biohackerspace in Renens, Switzerland, co-leading it together with Rachel Aronoff and the board,

Just to inform that we had a kit from the Odin at the lab. We decided to get in contact with the German authorities who suggested that our kit might also be contaminated. We sent them the kit.

We also got better informed on how our community could contribute
better to the DIY Bio movement. A more dynamic dialogue between the authorities and the affected peers of the DIY Bio movement would had been more desirable but we are now navigating between different landscapes with different time scales and communication protocols. We should push for a better more transparent ones, and of course our door is literally transparent and always open on Wednesdays or on appointment (basically cause we are volunteers and have side jobs or even lives!). Having a constructive and responsible attitude towards the issues we face could help shaping the science we dream of.

Best regards

Vanessa

El viernes, 22 de diciembre de 2017, 9:52:09 (UTC+1), Jonathan Ferooz escribió:
Nathan I think you are wrong, in Belgium we are very interested to collaborate with other groups (and I'm sure in other EU countries too). But in contrast with some other groups we are a small group with no funding.
What do you think we can do?
We are pushing, at our level, to show what is the DIYbio and hope that we'll can adapt our laws to facilitate the DIYbio.
In europe just have the right to put GFP in a genome it's a fight with the authorities.
If other groups want to collaborate with us in Belgium, we are open.
We are not making the show on TV, Nature or other, but yes, we are there.

Le jeudi 21 décembre 2017 23:20:55 UTC+1, Nathan McCorkle a écrit :




I am surprised this has got any more attention, given the lack of response the EU biohackers have provided here on this mailing list. It gives the impression that EU biohackers are not interested in collaboration, and are making noise for some self-serving goals. This behavior only makes me wary of EU biohackers. Very sad.


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-Nathan

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[DIYbio] Re: Freedom Evo Tecan Troubleshooting - HELP!

Hi Ana, 

I think I may be having a similar problem as you were last year. Did you ever figure out what was going on?? Did changing the AnswerTimeout parameter fix the problem? 

Thanks!! 

Joe 

On Monday, June 5, 2017 at 1:30:19 PM UTC-4, Ana Santos wrote:
Hi diybio community!

I was wondering if anyone out there has experience with the Freedom Evo Tecan Robot. 

We have ours for a few years (>5) and no one has good memories of working with it. And basically, we're just trying to use it to read 96 well plates (between 8 and 12) for 24h.

Everything starts out OK, it runs for about 6h and afterward it just stops working showing messages like "Your run has been stopped" or "Your run was aborted". No red light blinking. No alarm. 

However, if we hit the recover bottom, it re-starts working, as if nothing had happened. If we're really lucky, the run then proceeds until the end, but normally it keeps crashing systematically afterwards.

The only pattern we've been able to detect is that the robot normally stops working when the plate is inside the plate reader.

The technicians from Tecan are now almost part of the lab, because of all the problems we have with the Robot, but they never manage to tell us exactly is the problem. Whatever they do (switch a cable, tweak the software, plug/unplug all machinery) works for a couple of hours but we then go back to the same original problem.

We're just desperate to know if someone else has faced the same problem or if it is a problem with our machine.

I look forward to your feedback.

Best regards and DO GREAT SCIENCE!

Ana

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