Re: [DIYbio] Re: BioRad ICycler Password or Service Manual

11:40 PM |

Sadly no luck.  I haven't tried resetting the bios or using a brute force attack both are good suggestions from really good hackers. One day when I have more time I'll give it a shot. For now we have two working models in our lab in LA. 



On Oct 6, 2016, at 5:05 PM, Daniel Westcott <daniel.james.westcott@gmail.com> wrote:

Reviving this stale post. Did you have any luck? I just grabbed one of these from the surplus cart in our building's basement. It says Edge Fault or something. 


On Sunday, April 10, 2016 at 1:27:52 AM UTC-7, Roninlaw wrote:
Does anyone know the service option password for the BioRad ICycler or have a service manual?  I have two. One with a lid that won't heat and another with a heat block fault. I am hoping to have one good one by switching the lid. Or two if anyone has any experience or any ideas for replacing the peltiers.

Thanks,

Dan

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Re: [DIYbio] Re: DNA sequencer recommendations?

10:16 PM |

Thanks Bryan,

I just sent them a mail yesterday, their reply below:

It's still not all clear to me how long the cells last and how many times you can use them, still a newbie to this. Can I run say, a plant sample one day, then the next day a yeast sample, and so on... Or is it just for human DNA ? And how many days i can run alternating samples like this ? And is it for targeted sequencing ? then where do i get the primers ? Or the cell just reads everything we throw at it ? Replacement cells are not cheap based on the prices on their web site.

I would like to figure out how much it would cost me per year if say, i run 3-4 samples per month...

"How many samples you can run per for cell depends on your target region of interest/genome size and the depth of coverage you are looking to achieve per sample. On the newest R9.4 chemistry you can generate as much as 10 Gb of 1D data per flow cell, so you could use this as a starting point for your calculation. Although we ask that flow cells be returned after 8 weeks from receipt, bulk orders can be "drawn" upon as needed  - meaning that if you purchase 12 flow cells for example, you can schedule actual deliveries as needed for your experiments. This means that your remaining flow cells will automatically be updated to the latest chemistry as we continue to improve our pores, membranes and hardware."

On Wednesday, 26 October 2016 06:24:11 UTC+2, Bryan Jones wrote:

We just got a MinION at our biohacker space. The starter package is $1000. Haven't had a chance to play with it yet though.
https://nanoporetech.com/products/minion



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