That paper looks easy enough... the real investment is getting the
construct synthesized... just googling gene synthesis I found an offer
for $0.29/bp, so if you construct was 3000bp, that's $870
That's 100 people donating $10 each, not a terrible goal...
Someone mentioned due to inadequate enzyme cleanup, we might be able
to fish trade secret sequences out of commercial enzymes:
http://groups.google.com/group/diybio/browse_thread/thread/666d3b9f6cd439b2?fwc=1
(I couldn't find the older post I referenced in that thread, I'll try
searching some more)
What would a good set of enzymes for bootstrapping be? How much
synthesis do we need to buy before having a nice toolkit?
On Mon, Nov 14, 2011 at 4:24 PM, Robert O'Callahan <ropoctl@rice.edu> wrote:
> The DIY bio community definitely needs cheaper tools and reagents. Would
> anyone have interest in having some strains suitable for the easy expression
> and purification of restriction enzymes, polymerases, and other useful ones?
> The method in this paper
> http://www.sciencedirect.com/science/article/pii/S1369703X11000325 seems
> pretty well suited for this. Another potential money saver would be a
> publicly available set of plasmids easily digested into quality DNA ladders.
> $50 per tube of restriction enzyme and $1 per lane of commercial ladders
> are not individually huge costs, but making these easy for individual to
> produce for themselves might lessen the barriers to entry.
> -Rob
>
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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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