Re: Get a plasmide - buy? share?

As with what Averie said, freezing is easy with DNA. Just put it in the
freezer, surround with thermal mass, and leave it alone.

However, if you'll be using the DNA within the month, it's probably best
*not* to freeze it..*if* it comes in "Tris-EDTA" buffer. This is
commonly shortened to TE. TE buffer protects DNA from nucleases and
maintains a pH that enhances stability for extended periods at 4C
instead; no freezing required.

The reason to opt for no-freeze for short periods is that DNA is harmed
by freeze and thaw, so you don't want to be freezing it today only to be
thawing it for use tomorrow.

On 21/12/11 22:39, Mega wrote:
> When I get the plasmids, how long can I store them in the freezer
> (-20°C)?
> Or do I have to store them at -80°C?
>
> Do I have to add something to the plasmids so they don't get damaged
> by freezing??
>
>
>
>
>

--
www.indiebiotech.com
twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.