Re: Get a plasmide - buy? share?

> I've been trying to find my notes on what I did, or the paper itself,
> and I can't seem to find either on my HDD just now.
>
> I'd suggest 200ul-400ul, with 20ul-40ul DNA, off the top of my head.

Here's the paper. Full text is free on Pubmed Central.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC286873/?tool=pmcentrez&rendertype=abstract

Looks like they used 0.1mL of cells in TSS and 100pg of DNA. But
since there is no heating step, any volume of cells should work just
fine.

-cory

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