First off, greetings! I have been lurking for a bit, but this is my
first post here.
Thank you all for the valuable information in this thread. I am still
trying to get my lab set up to do basic transformations. One thing I
am curious about is purification of plasmids so that I can continue to
use the plasmids that I have. Looking for miniprep columns online, I
am unable to locate a seller that ships to residential addresses. Is
there a way to circumvent that? Also, specifically to Cathal Garvey,
you mentioned that you could "roll your own" in the context of
columns. Do you have any more information on this? I would like to
keep costs to a minimum, so making my own equipment is definitely of
interest to me.
Additionally, I have a general question about transformations (I have
done a few before, but I didn't have full details on the strains of
bacteria at the time). When performing a transformation, is it
necessary to work with a strain that is deficient in restriction
modification, or is that only an issue if you are working with a fully
synthetic sequence (i.e. just purchased from a DNA synthesis company)?
Thank you, everyone!
On Dec 21, 6:08 am, Cathal Garvey <cathalgar...@gmail.com> wrote:
> I've been trying to find my notes on what I did, or the paper itself,
> and I can't seem to find either on my HDD just now.
>
> I'd suggest 200ul-400ul, with 20ul-40ul DNA, off the top of my head.
>
> On 20/12/11 16:01, Mac Cowell wrote:
>
> > What volume of cells is used in step 3?
>
> > 231.313.9062 // @100ideas // sent from my rotary phone
>
> --www.indiebiotech.com
> twitter.com/onetruecathal
> joindiaspora.com/u/cathalgarvey
> PGP Public Key:http://bit.ly/CathalGKey
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