[DIYbio] Re: Recycling Antibodies for Immunocytochemistry

Oh, nice idea. Protein recycling; that's one step ahead of the curve!

If the antibody was purified using some peptide-based method, such as
maltose-binding proteins or his-tags, you could try using the same
methods to separate from the finished experiment?

On 18/01/12 16:48, Andrea J Bae wrote:
> Hello Everyone!
> I'm an undergrad at Wellesley College starting immunocytochemistry.
> I'd like to know if there are ways to recycle antibodies for ICC. Does
> anybody have any suggestions from experience or any protocol
> suggestions from the literature?
>
> Thanks!
> Andrea
>


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