Re: Antibiotic Resistance Concerns (Was: Re: ...biolumineszent plant)

http://www.livescience.com/10307-transplanted-genes-plants-light.html

The company wanted to sell autoluminescent plants... But google has no
good results for 'Bioglow inc"
Else it maybe would have been possible to make contact and by a
prototype


On 14 Jan., 21:13, Nathan McCorkle <nmz...@gmail.com> wrote:
> the table here lists the working concentration of ampicillin to
> commonly be 100ug/mL, kanamycin @ 50ug/mL:http://www.qiagen.com/plasmid/bacterialcultures.aspx#tab3
>
> so you'd mix up a stock solution of ampicillin (they recommend
> 50mg/mL)... so in 50mL of distilled, sterile water, add 2.5 g
> ampicillin
>
> if you make 250mL of LB agar solution, after its been sterilized and
> allowed to cool to less than 55 degrees C, you'd add (250mL*100ug/ml)
> 25mg ampicillin. Since your stock solution was 50mg/mL, you'd add
> 0.5mL of this stock solution to the media
>
>
>
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>
>
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>
> On Sat, Jan 14, 2012 at 12:07 PM, Mega <masterstorm...@gmail.com> wrote:
>
> > Ok,
>
> > I got another question:
>
> > After transformation of the bactieria I have to plate them on lb +
> > agar + ampicilline.
>
> > How much ampicilline do I have to get for one petri dish??
>
> > Thx
>
> > --
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> --
> Nathan McCorkle
> Rochester Institute of Technology
> College of Science, Biotechnology/Bioinformatics

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