[DIYbio] Re: Plasmids: Best before?

Thanks!!

On 29 Feb., 11:27, Cathal Garvey <cathalgar...@gmail.com> wrote:
> In T.E. buffer, DNA is pretty stable for months at 4c. Indeed, some schools of thought suggest that freezing is more harmful, if using buffers containing salt (like TE).
>
> In water, far less stability is expected, but you'll probably still get transformants.
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> Mega <masterstorm...@gmail.com> wrote:
> >Hello,
>
> >I was wondering how long I could still use the plasmids I have.
>
> >They were stored around 2 month in the freezer (-20°C) and now some 6
> >weeks in the fridge (~7°C).
>
> >Will they degrade? And, if they degrade, when only half of them will
> >not be degraded (you can work with the half available, although you'll
> >get smaller yields).
>
> >Best
>
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