just use imageJ... there's gel lane analysis built-in, it gives you an
intensity plot...
On Wed, Feb 1, 2012 at 11:54 AM, Jeswin <phillyj101@gmail.com> wrote:
> I'm very bad at estimating things. How important is the value of the
> intensity when I calculate how much insert to use for ligation?
>
> I run 1 uL of my sample and 1 uL of ladder on a gel.
> For the look of things, lane 3 insert looks about the same as the
> plasmid intensity and lane 4 insert looks double the insert.
>
> Thanks
>
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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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