I want to extract DNA from fresh/dried plant material. One technique is to macerate the tissue by placing it in a tube with beads and mounting it on a vortexer. There are commercial kits (http://www.geneworks.com.au/Product/ProductDetail.aspx?p=32&pid=9582) which require adaptors for some vortexers (http://www.mobio.com/vortex-and-vortex-adapters/vortex-adapters-for-vortex-genie-2.html). I believe a dremelfuge tube holder mounted on a vortexer (mine is 1.5"/38mm dia) would work as well. My thoughts were to get a piece of 1.5" plastic tube to fit over the vortexer hub and cut slots in the top to correspond with the radial arms of the dremelfuge. The tubes may need restraint to avoid being shaken out (maybe a plastic wedge between the cap and the hub). I haven't tried any of these ideas yet - still looking for a suitable piece of plastic tube.
I am not familiar with your technique, do you have any more details
about it? Maybe I can offer some ideas.
On Jan 20, 2:26 am, David McWilliam <dmcwill...@gmail.com> wrote:
> Great post. I've been looking for something like this for macerating plant
> material in bead beater tubes prior to DNA extraction. I've just bought a
> dremelfuge which arrived today and am thinking of ways to attach it to a
> vortexer. The horizontal tube position would help maximise the bead
> vortexing although it may need something to stop the tube caps from
> popping. Maybe a vortexer modification of the dremelfuge - Cathal?
> DMcW
>
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit this group at http://groups.google.com/group/diybio?hl=en.
0 comments:
Post a Comment