I asked the proffessor if I could have access to the lab to do a
transformation. And guess what.
Monday I'm doing it.
But there are still a few questions...
When I make an LB Amp Agar Dish, I first mix water and LB Agar. Then I
heat it up. I think making 50 ml will be enough for one or two petri
dish.
Then I have to sterilize it. Will 3 mins in the microwave do the job,
or will I need to put it into the autoclaveur for 20mins as
recommended?
And, at <55°C I have to add the ampicillin which is not heat
ressistant.
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