On Mar 1, 2012 1:58 PM, "Mega" <masterstorm123@gmail.com> wrote:
>
> reg. the plates:
> http://www.benchfly.com/video/164/how-to-pour-lbagar-plates/
> This video was very helpful (second vid)
>
>
>
> Thank you for your help!!
>
> Well, I could either dose the LB medium as it is said on the LB-Agar
> package:
> 10g LB-Agar per liter. I don't heat it, so the agar will sink to the
> ground. The supernatant is then LB-water.
>
> Or I just use 'a lot' of LB-Agar (without calculating) and add some
> water. What will stay at the ground of the tube is then agar mixed
> with undissolved LB. (Not efficient, but for very small dosages maybe
> ok)
Huh?
You need to heat agar, or it won't dissolve and would be pointless to add. Don't use too much or the salt concentration will be wrong, why wouldnt you just use what the package specifies?
Or, are you saying you don't have LB without agar already added?
>
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