Re: [DIYbio] Miniprep without EDTA

Tried to get diat. earth at a store. They didn't have it.

Another store, the Lagerhaus (don't know if there's an equivalent in english, mainly farmers buy there, but also private people)  had already closed when I came from university.



do you think, in university we will have some of it? Anyway, tomorrow I'll ask there (although I don't know what university would do with it...)

2012/5/29 Jeswin <phillyj101@gmail.com>

On Mon, May 28, 2012 at 1:08 PM, Andreas Sturm <masterstorm123@gmail.com> wrote:
> So tomorrow I'll go get some diatomaeous earth and some KCl....
>
>
> When I do it without a liquid media overnight culture (from agar directly),
> how many bacteria do I have to take with the inoculation loop?
>

Minipreps use bacteria grown in liquid media. I guess there is a
higher density of cells in liquid so that you get better DNA
purification efficiency.

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