Re: [DIYbio] Miniprep without EDTA

On Wed, May 30, 2012 at 5:52 AM, Mega <masterstorm123@gmail.com> wrote:
> Was at a swimming pool store. In former times it was used, but now they sell nano powder.
>
>
> I put the glowing bacteria into a marmelade jar containing some 200-300 mL of LB-Medium. It was glowing for some days but now it's not glowing anymore... I assume most of the bacteria are dead. Can I still use them for miniprep, or are the plasmids destroyed when doing apoptosis???
>

First do you mean glowing or fluorescing, we need to be consistent
with terminology here.

I've purified GFP protein from cell lysate, and it still fluoresced,
so it doesn't depend on cell's being alive, but certainly a lysed cell
would have proteases all over the place and the GFP could then degrade
rapidly


--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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