Hi L,
I didn't quite understand your question-are you looking for protocols to transform yeast cells with the artificial chromosome or are you looking for protocols for cloning large pieces of DNA into artificial chromosome vectors?
On Monday, June 25, 2012 8:27:31 PM UTC-4, L wrote:
Hey there everyone,--
I'm looking for a way to quickly create protocols for transformation of artificial yeast chromosomes. Got lots of ideas, the means to try them all, and now I'm just looking into what restriction enzymes I'll need. Confused by BLAST, any help using that would be awesome.
Thank you!
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