Re: [DIYbio] How is Agrobacterium Tumefaciens grown? Is it possible to find in soil?

hmmm...
I live in Ireland but would be going over to Indiana in August, I don't know if that's any help...it'd leave all the importation ('smuggling') up to me. I could send you the address if you have a e-mail?
Is it only found over there or is this world-wide anyway?

On Monday, June 18, 2012 7:09:52 AM UTC+1, cory....@gmail.com wrote:

> This wonderful 'stuff' seems to be the 'gold dust' for doing genetics with:)
> Does anybody know anything about it? Like where to find it, what conditions
> and so on it likes.....How to encourage it?

You can actually grow it on LB just like E. coli, although if you put
it in a 37C incubator it will die.  It grows best on LB at 27C.


> Would love to know, even have somebody mail me a sample...maybe:)

If you're in California then you're in luck.  I can send you a sample
no problem.  If you're in the US but outside California, get a permit
for interstate transportation of plant pathogens here:
http://www.aphis.usda.gov/plant_health/permits/organism/plant_pathogens.shtml
and I'll send a sample.  If you're outside the US, check the
regulations in your country and get the proper permits.

Summary:  transporting plant pathogens across political boundaries is
complicated, even if it's a "disarmed" strain like GV3101.  Your best
bet is to find a plant genetics lab at a university near you and ask
the professor for a sample.


-cory

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