Re: [DIYbio] How is Agrobacterium Tumefaciens grown? Is it possible to find in soil?

I see a body cavity search in his future. I think he is trying to get it in the us and bring it back to Ireland. Now you know who to blame for the next potato famine.



On Jun 29, 2012, at 8:12 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:

> I hope you're not suggesting importing a _wild_ strain of agrobacterium into the US? That'd be morally and legally a 'bad thing'.
>
> Barra Darcy <baradarcy@gmail.com> wrote:
>
>> hmmm...
>> I live in Ireland but would be going over to Indiana in August, I don't
>>
>> know if that's any help...it'd leave all the importation ('smuggling')
>> up
>> to me. I could send you the address if you have a e-mail?
>> Is it only found over there or is this world-wide anyway?
>>
>> On Monday, June 18, 2012 7:09:52 AM UTC+1, cory....@gmail.com wrote:
>>>
>>>> This wonderful 'stuff' seems to be the 'gold dust' for doing
>> genetics
>>> with:)
>>>> Does anybody know anything about it? Like where to find it, what
>>> conditions
>>>> and so on it likes.....How to encourage it?
>>>
>>> You can actually grow it on LB just like E. coli, although if you put
>>
>>> it in a 37C incubator it will die. It grows best on LB at 27C.
>>>
>>>
>>>> Would love to know, even have somebody mail me a sample...maybe:)
>>>
>>> If you're in California then you're in luck. I can send you a sample
>>
>>> no problem. If you're in the US but outside California, get a permit
>>
>>> for interstate transportation of plant pathogens here:
>>>
>>>
>> http://www.aphis.usda.gov/plant_health/permits/organism/plant_pathogens.shtml
>>
>>> and I'll send a sample. If you're outside the US, check the
>>> regulations in your country and get the proper permits.
>>>
>>> Summary: transporting plant pathogens across political boundaries is
>>
>>> complicated, even if it's a "disarmed" strain like GV3101. Your best
>>
>>> bet is to find a plant genetics lab at a university near you and ask
>>> the professor for a sample.
>>>
>>>
>>> -cory
>>>
>>
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