Re: [DIYbio] Re: Any yeast culturing and/or gen transfer protocol, simple enough to do at a simple laboratory and sourcing yeast?

If you have the sequence, try plasmapper.  It does a pretty sweet visual and textual annotation.

--A

On Jun 26, 2012 6:19 PM, "Cathal Garvey" <cathalgarvey@gmail.com> wrote:

Most plasmids for Agrobacterium are E.coli-compatible, they have origin
sites for both. E.coli is used when assembling the new construct, and
Agrobacterium is used to transfer the DNA to plants.

If that's so, start out by hacking it into E.coli with an appropriate
antibiotic, and then you can make as much as you need for working with
Agrobacterium.

Run the plasmid sequence through BLASTn, and you should find a region
that's homologous to the origin on lots of E.coli plasmids.. or post the
plasmid map, and perhaps it's labelled?

On 26/06/12 15:52, Andreas Sturm wrote:
> Ok, thank you!!
>
> So probably it's not the best idea to try it with a rare/expensive plasmid
> :D
>
>
>
> 2012/6/26 Cathal Garvey <cathalgarvey@gmail.com>
>
>> Don't know, sorry! :)
>>
>>
>

--
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