Re: [DIYbio] Re: Genomic integration of DNA

Phage transduction is a pretty tedious and slow process.  I like the flp-frt system for homologous recombination.  I've only used it in E. coli K12, but there are many papers about the use of this method across a wide range of species.  The methodology is fairly simple and straight forward and plasmids are available from an open-source depository.  It can get tricky when integrating into a site that is resistant to recombination, but that usually for a good reason

http://www.igmm.cnrs.fr/IMG/pdf/flpin_pog44_man-2.pdf

http://www.pnas.org/content/93/12/6191.short

On Thursday, May 31, 2012 11:56:27 AM UTC-7, Mega wrote:

About the Red-System (Red - lambda phage):

It works just for E.Coli and specifically only in E.Coli K12 ??

So it can't be used to engineer Cyanobacteria, B.Subrilis, etc. ??


Are there ready-to use systems (you just buy and use) for those organisms??


Thx,

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