[DIYbio] Re: Free plasmids for amateur biologists.

Great discovery!!!

Did I understand the mechanism of those plasmids correctly?

You transform bacteria with the pBluescript (derivate). And select for it.

Then, all you have to do is put a helper phage on the petri dish.

The sequence is then inserted. 

(?)









Am Samstag, 30. Juni 2012 13:03:47 UTC+2 schrieb Dorif:

Hello! I have found the way to get some free plasmids with map and work protocol. The Aldevron company distributes free samples of pRc/CMV-Hbs(S) and pCMVHB-S2.S plasmids. Both are based on pBlueScript plasmid and contain Ampicilline resistance gene. Each sample contains 2-4 ug of plasmid DNA. Samples are delivered via postage service. Link to order samples: http://www.aldevron.com/products/dnas/plasmids/ 

I hope it'll be useful for somebody.

P.S.: sorry for my bad English.)

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