You should not work with wild strains of ecoli. It is way too dangerous. It is best to wait until you can get the safe strain from a local university or supplier.
I know.) But in Europe are located antibody producing facilities(Aldevron Freiburg, former Genovac), DNA production is located in Fargo, North Dacota(from where i received my plasmids).And I have two questions: a) from where can I obtain E.coli strain, suitable for transformation with these plasmids(of course, I have wild strains of E.coli and plasmids are in fridge(so, I can wait a little), but...), b) With what I can replace TrisEDTA beffer, recommended for plasmid extraction from samples(Currently I have no Good's buffers at all, but I have wide range of inorganic compounds, suitable for PBS, potassium-phosphate buffer, water, acetate buffer, oxalate buffer)?--
воскресенье, 1 июля 2012 г., 22:55:46 UTC+3 пользователь Nathan McCorkle написал:Nice find! They're based in the U.S. but also have a location in Germany too.
On Sat, Jun 30, 2012 at 7:03 AM, Александр Дориф <dorif11@gmail.com> wrote:
> Hello! I have found the way to get some free plasmids with map and work
> protocol. The Aldevron company distributes free samples of pRc/CMV-Hbs(S)
> and pCMVHB-S2.S plasmids. Both are based on pBlueScript plasmid and contain
> Ampicilline resistance gene. Each sample contains 2-4 ug of plasmid DNA.
> Samples are delivered via postage service. Link to order
> samples: http://www.aldevron.com/products/dnas/plasmids/
>
> I hope it'll be useful for somebody.
>
> P.S.: sorry for my bad English.)
>
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Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics
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