Re: [DIYbio] Re: Artificial Jellyfish

Oh, that is *very* clever. My apologies, I was only running off what I
saw in the email thread! :)

On 31/07/12 22:04, Nathan McCorkle wrote:
> On Tue, Jul 31, 2012 at 4:59 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
>> IMHO, Sugar would make a poor substrate for cell culture. The osmotic
>> pressure of growing on sugar would be huge; that's one reason why it's
>> traditional to add so much sugar to preserves like jam.
>>
>
> Cathal, they don't use the sugar to grow on, they print a mold with
> the sugar, then add gelatin + cells and pour it over the mold. Once
> the gelatin hardens, the mass is rinsed in an orbital shaker with
> media or buffer (not sure which) to dissolve the sugar, then that
> liquid is replaced with media. In Jordan's case, the mold looks like
> vasculature to aid perfusion later (he perfuses with orbital shaking,
> but I think he's also working on using a pump driven system).
>

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