Re: [DIYbio] (transfected) Insect cell -> entire organism

yes you want to hit the germline to get expression in the whole
organism (or at least to get the new DNA in the whole organism, you
may add DNA that only expresses in one body area)

theoretically you could also do somatic cell nuclear transfer (SCNT)
from a modified cell to an embryo

On Sun, Jul 29, 2012 at 8:25 AM, Mega <masterstorm123@gmail.com> wrote:
> Hey,
>
> I read that it would be quite easy to add genes to insect cells using the
> piggyBac transposon. Frequently used for recombinant protein expression in
> insect cell lines.
> So I was wondering how you could get an insect out of those cells. Do you
> have to transfect insect sperm? Insects are not plants, so you probably
> can't use any cell for reproducing an entire organism?
>
>
>
>
>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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