Re: [DIYbio] (transfected) Insect cell -> entire organism

Not so much complicated as extremely difficult. You need a
micromanipulator and microneedle for one thing, and then you need the
manual skill to use one effectively. Finally, you need lots and lots of
insect ova! :)

This is routine for making transgenic fruit flies, for example. I was
interested in trying it with sea monkies (Artemia salina) for a while
until I discovered how hard it is to get microneedles/micromanipulators.
I'm leaving it for later!

On 30/07/12 15:56, Andreas Sturm wrote:
>> theoretically you could also do somatic cell nuclear transfer (SCNT)
>>from a modified cell to an embryo
>
>
> Of course, but this seems even more complicated ;)
>
>
>
> 2012/7/30 Nathan McCorkle <nmz787@gmail.com>
>
>> yes you want to hit the germline to get expression in the whole
>> organism (or at least to get the new DNA in the whole organism, you
>> may add DNA that only expresses in one body area)
>>
>> theoretically you could also do somatic cell nuclear transfer (SCNT)
>> from a modified cell to an embryo
>>
>> On Sun, Jul 29, 2012 at 8:25 AM, Mega <masterstorm123@gmail.com> wrote:
>>> Hey,
>>>
>>> I read that it would be quite easy to add genes to insect cells using the
>>> piggyBac transposon. Frequently used for recombinant protein expression
>> in
>>> insect cell lines.
>>> So I was wondering how you could get an insect out of those cells. Do you
>>> have to transfect insect sperm? Insects are not plants, so you probably
>>> can't use any cell for reproducing an entire organism?
>>>
>>>
>>>
>>>
>>>
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>>
>>
>>
>> --
>> Nathan McCorkle
>> Rochester Institute of Technology
>> College of Science, Biotechnology/Bioinformatics
>>
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