Re: [DIYbio] extracting plant textile DNA

On Thu, Jun 21, 2012 at 8:07 AM, sharvari deshpande
<sharvari.deshpande996@gmail.com> wrote:
> I will be using 3 different protocols for extraction , let see which one is
> better .
> you are talking about some inhibitors , what kind of inhibitors ???????
>

So how did the extractions go? Did you get the DNA?

I was able to get good DNA from a sweater and unspun textile material.
We amplified and cloned the regions and send it for sequencing. It was
a mysterious process where sometimes we get the band we want on the
gel but then it doesn't work again.

On Thu, Jun 21, 2012 at 9:29 AM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
> Polysaccharides in general are a common inhibitor of PCR, so yea; you'd
> need to purify out any that made their way through the DNA extraction steps.

Turns out I didn't need to do additional extraction. The Qiagen plant
DNeasy was pretty good.

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