Re: [DIYbio] Open Gelbox/Transilluminator Source Files

I think Avery meant "blue LEDs off ebay"

On Thu, Aug 2, 2012 at 7:57 PM, Avery louie <inactive.e@gmail.com> wrote:
> Get some blue less off ebay. Works very well with gelgreen. Go to
> www.bosslab.org if you want to see pictures.
>
> On Aug 2, 2012 6:24 PM, "AMD" <amdurbin@gmail.com> wrote:
>>
>> OP wanted a blue light transilluminator, not a UV transilluminator.
>>
>> On Tuesday, June 5, 2012 7:18:52 AM UTC-7, Pieter wrote:
>>>
>>> The full instructions can be found on instructables:
>>> -
>>> http://www.instructables.com/id/Gel-electrophoresis-system-mini/?ALLSTEPS
>>> - http://www.instructables.com/id/UV-Transilluminator/?ALLSTEPS
>>>
>>> Following these steps would probably also lead you to a fine gelbox:
>>> http://learn.genetics.utah.edu/content/labs/gel/build_gel_box.pdf
>>>
>>> The instructables are pretty nice, but it is sometimes hard to get all
>>> the components. Perhaps we should make a kit, as a supplement to the OpenPCR
>>>
>>> On Tuesday, 5 June 2012 10:43:01 UTC+2, cory....@gmail.com wrote:
>>>>
>>>> I don't know anything about the Open Gelbox 2.0 but IORodeo has a gel
>>>> box which they give away the design files for (
>>>> http://www.iorodeo.com/content/mini-gel-electrophoresis-system )
>>>> including the spec sheet, Open SCAD files and laser-cutting files.
>>>> They also have python scripts in one of their Bitbucket repositories (
>>>> https://bitbucket.org/iorodeo/transilluminator_enclosure/src ) which
>>>> allow you to input different dimensions and it will generate the SCAD
>>>> files automatically. So if you want a larger or smaller box than the
>>>> default, you can easily make that happen.
>>>>
>>>> As for the blue and amber filters for an LED illuminator, I had good
>>>> luck using gel filters (the colored things you see covering lights at
>>>> theater performances or movie sets). I got them from this guy on ebay
>>>>
>>>> http://www.ebay.com/itm/390061292911?ssPageName=STRK:MEWAX:IT&_trksid=p3984.m1423.l2649
>>>> They have a huge selection of colors and they are very cheap ($6 for
>>>> 4sq ft). If you want I can go through my notebook and find which
>>>> specific colors worked best.
>>>>
>>>> No need to use an arduino to control the LEDs. If you have a DC power
>>>> supply, hook a few LEDs in series with a resistor and add a switch if
>>>> you want. Much cheaper.
>>>>
>>>> Also, if you're interested in making a UV illuminator, IORodeo has
>>>> design files for that as well
>>>> http://www.iorodeo.com/content/transilluminator-enclosure
>>>>
>>>>
>>>> -cory
>>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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