Re: [DIYbio] Re: Building a particle bombardement device DIY

http://en.wikipedia.org/wiki/Magnetofection

On Aug 27, 2012 2:35 PM, "Patrik D'haeseleer" <patrikd@gmail.com> wrote:

That's a great find!

I think we'll just have to experiment a bit to find the right particle composition. Obviously, you want something that is relatively inert, preferably high density, and very small particle size. So for a professional lab, gold or tungsten nanoparticles are an obvious choice. That does *not* mean this is the only way to get it to work though!

I brought up magnetite particles before, because I'd been looking into how to make ferrofluid lately. It turns out there's a simple chemical route to make magnetite nanoparticles that are way smaller than the magnetite powder I had bought online in initially. And magnetite may not be as heavy as gold, but I can tell you that jar of magnetite has some serious heft to it. Probably worth a try. Plus, the magnetic nature of the particles may allow us to manipulate them in ways you can't do with gold.

Patrik

On Monday, August 27, 2012 8:38:46 AM UTC-7, Mega wrote:

I was thinking in investing into http://www.alibaba.com/product-gs/226547546/Portable_Gene_Gun.html this one. 300$ for one device.


But if the particles are this expensive...

On Mon, Aug 27, 2012 at 12:22 PM, Patrik D'haeseleer <pat...@gmail.com> wrote:

Here's some more useful DIY info:

http://thalamus.wustl.edu/nonetlab/ResourcesF/genegun/Genegun.htm
http://dictybase.org/techniques/particle%20gun/particle_gun.htm
http://diagnosisp.com/dp/journals/view_pdf.php?journal_id=1&archive=0&issue_id=5&article_id=148

On Monday, August 27, 2012 3:03:45 AM UTC-7, Patrik D'haeseleer wrote:

We were tossing around the idea of a DIY gene gun at BioCurious as well. Sure, helium and gold particles may be more efficient, but I bet a hacked bb gun and, say, magnetite particles may give at least some transformation as well.

Here's a great video on how to prepare DNA "bullets", and how to use the gene gun to transform a small selection of cells in live neuronal samples:

http://www.jove.com/index/details.stp?id=675

Patrik

On Sunday, August 26, 2012 3:15:53 AM UTC-7, Mega wrote:

Hi,

I 'm wondering how one could build such a device for particle bombardement.

They usually use Helium, but why not Nitrogen or Carbon dioxide (widely available). I read they use low pressure, but what is low?
Does it have to be in a vakuum??? I can do this, but it will be expensive and difficult to build the vakuum-tight casing and buy the vakuum pump.

Can there be too much  pressure (so the particles fly through the cells without leaving DNA)? Or can I configure it just using 'some pressure' ?


(I have to look up the laws of my country, but as you cannot harm anyone with this device, it shouldn't be an issue)

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