Re: [DIYbio] Re: Building a particle bombardement device DIY

Magnetofection's article reads like an advert.

Also, if the article is to be believed, it sounds like it's more or less
100% based on chemical transformation, with the "magneto" part only
describing the means of concentrating the transforming material close to
the cells. The particles are otherwise just DNA coated in "proprietary
formulations", probably some blend of PEG and molecules with some
affinity for cell surface antigens.

On 28/08/12 18:52, Nathan McCorkle wrote:
> you could also try magnetofection or magnetoporation... some term like
> that...
>
> http://en.wikipedia.org/wiki/Magnetofection
>
>
> On Aug 27, 2012 2:35 PM, "Patrik D'haeseleer" <patrikd@gmail.com> wrote:
>
>> That's a great find!
>>
>> I think we'll just have to experiment a bit to find the right particle
>> composition. Obviously, you want something that is relatively inert,
>> preferably high density, and very small particle size. So for a
>> professional lab, gold or tungsten nanoparticles are an obvious choice.
>> That does *not* mean this is the only way to get it to work though!
>>
>> I brought up magnetite particles before, because I'd been looking into how
>> to make ferrofluid lately. It turns out there's a simple chemical route to
>> make magnetite nanoparticles that are way smaller than the magnetite powder
>> I had bought online in initially. And magnetite may not be as heavy as
>> gold, but I can tell you that jar of magnetite has some serious heft to it.
>> Probably worth a try. Plus, the magnetic nature of the particles may allow
>> us to manipulate them in ways you can't do with gold.
>>
>> Patrik
>>
>> On Monday, August 27, 2012 8:38:46 AM UTC-7, Mega wrote:
>>>
>>> I was thinking in investing into http://www.alibaba.com/**
>>> product-gs/226547546/Portable_**Gene_Gun.html<http://www.alibaba.com/product-gs/226547546/Portable_Gene_Gun.html>this one. 300$ for one device.
>>>
>>>
>>> But if the particles are this expensive...
>>>
>>>
>>>
>>>
>>>
>>> On Mon, Aug 27, 2012 at 12:22 PM, Patrik D'haeseleer <pat...@gmail.com>wrote:
>>>
>>>> Here's some more useful DIY info:
>>>>
>>>> http://thalamus.wustl.edu/**nonetlab/ResourcesF/genegun/**Genegun.htm<http://thalamus.wustl.edu/nonetlab/ResourcesF/genegun/Genegun.htm>
>>>> http://dictybase.org/**techniques/particle%20gun/**particle_gun.htm<http://dictybase.org/techniques/particle%20gun/particle_gun.htm>
>>>> http://diagnosisp.com/dp/**journals/view_pdf.php?journal_**
>>>> id=1&archive=0&issue_id=5&**article_id=148<http://diagnosisp.com/dp/journals/view_pdf.php?journal_id=1&archive=0&issue_id=5&article_id=148>
>>>>
>>>>
>>>>
>>>>
>>>> On Monday, August 27, 2012 3:03:45 AM UTC-7, Patrik D'haeseleer wrote:
>>>>>
>>>>> We were tossing around the idea of a DIY gene gun at BioCurious as
>>>>> well. Sure, helium and gold particles may be more efficient, but I bet a
>>>>> hacked bb gun and, say, magnetite particles may give at least some
>>>>> transformation as well.
>>>>>
>>>>> Here's a great video on how to prepare DNA "bullets", and how to use
>>>>> the gene gun to transform a small selection of cells in live neuronal
>>>>> samples:
>>>>>
>>>>> http://www.jove.com/index/**deta**ils.stp?id=675<http://www.jove.com/index/details.stp?id=675>
>>>>>
>>>>> Patrik
>>>>>
>>>>> On Sunday, August 26, 2012 3:15:53 AM UTC-7, Mega wrote:
>>>>>>
>>>>>> Hi,
>>>>>>
>>>>>> I 'm wondering how one could build such a device for particle
>>>>>> bombardement.
>>>>>>
>>>>>> They usually use Helium, but why not Nitrogen or Carbon dioxide
>>>>>> (widely available). I read they use low pressure, but what is low?
>>>>>> Does it have to be in a vakuum??? I can do this, but it will be
>>>>>> expensive and difficult to build the vakuum-tight casing and buy the vakuum
>>>>>> pump.
>>>>>>
>>>>>> Can there be too much pressure (so the particles fly through the
>>>>>> cells without leaving DNA)? Or can I configure it just using 'some
>>>>>> pressure' ?
>>>>>>
>>>>>>
>>>>>> (I have to look up the laws of my country, but as you cannot harm
>>>>>> anyone with this device, it shouldn't be an issue)
>>>>>>
>>>>>>
>>>>>> --
>>>> You received this message because you are subscribed to the Google
>>>> Groups "DIYbio" group.
>>>> To post to this group, send email to diy...@googlegroups.com.
>>>> To unsubscribe from this group, send email to diybio+un...@**
>>>> googlegroups.com.
>>>> To view this discussion on the web visit https://groups.google.com/d/**
>>>> msg/diybio/-/uzJTC0fj9DMJ<https://groups.google.com/d/msg/diybio/-/uzJTC0fj9DMJ>
>>>> .
>>>>
>>>> For more options, visit https://groups.google.com/**groups/opt_out<https://groups.google.com/groups/opt_out>
>>>> .
>>>>
>>>>
>>>>
>>>
>>> --
>> You received this message because you are subscribed to the Google Groups
>> "DIYbio" group.
>> To post to this group, send email to diybio@googlegroups.com.
>> To unsubscribe from this group, send email to
>> diybio+unsubscribe@googlegroups.com.
>> To view this discussion on the web visit
>> https://groups.google.com/d/msg/diybio/-/nhN46z8-BcMJ.
>> For more options, visit https://groups.google.com/groups/opt_out.
>>
>>
>>
>

--
www.indiebiotech.com
twitter.com/onetruecathal
joindiaspora.com/u/cathalgarvey
PGP Public Key: http://bit.ly/CathalGKey

--
You received this message because you are subscribed to the Google Groups "DIYbio" group.
To post to this group, send email to diybio@googlegroups.com.
To unsubscribe from this group, send email to diybio+unsubscribe@googlegroups.com.
For more options, visit https://groups.google.com/groups/opt_out.