Re: [DIYbio] Re: Building a particle bombardement device DIY

On Tue, Aug 28, 2012 at 2:33 PM, Cathal Garvey <cathalgarvey@gmail.com> wrote:
> Magnetofection's article reads like an advert.

hmm, i don't see it that way

>
> Also, if the article is to be believed, it sounds like it's more or less
> 100% based on chemical transformation, with the "magneto" part only

I also don't see it that way... " Their association with the gene
vectors (DNA, siRNA, ODN, virus, etc.) is achieved by salt-induced
colloidal aggregation and electrostatic interaction" then you apply an
electromagnetic field and the magnet:DNA complex rips into the cell.

More like electroporation or gene gun to me than chemical methods

> describing the means of concentrating the transforming material close to
> the cells. The particles are otherwise just DNA coated in "proprietary
> formulations", probably some blend of PEG and molecules with some
> affinity for cell surface antigens.
>
> On 28/08/12 18:52, Nathan McCorkle wrote:
>> you could also try magnetofection or magnetoporation... some term like
>> that...
>>
>> http://en.wikipedia.org/wiki/Magnetofection
>>
>>
>> On Aug 27, 2012 2:35 PM, "Patrik D'haeseleer" <patrikd@gmail.com> wrote:
>>
>>> That's a great find!
>>>
>>> I think we'll just have to experiment a bit to find the right particle
>>> composition. Obviously, you want something that is relatively inert,
>>> preferably high density, and very small particle size. So for a
>>> professional lab, gold or tungsten nanoparticles are an obvious choice.
>>> That does *not* mean this is the only way to get it to work though!
>>>
>>> I brought up magnetite particles before, because I'd been looking into how
>>> to make ferrofluid lately. It turns out there's a simple chemical route to
>>> make magnetite nanoparticles that are way smaller than the magnetite powder
>>> I had bought online in initially. And magnetite may not be as heavy as
>>> gold, but I can tell you that jar of magnetite has some serious heft to it.
>>> Probably worth a try. Plus, the magnetic nature of the particles may allow
>>> us to manipulate them in ways you can't do with gold.
>>>
>>> Patrik
>>>
>>> On Monday, August 27, 2012 8:38:46 AM UTC-7, Mega wrote:
>>>>
>>>> I was thinking in investing into http://www.alibaba.com/**
>>>> product-gs/226547546/Portable_**Gene_Gun.html<http://www.alibaba.com/product-gs/226547546/Portable_Gene_Gun.html>this one. 300$ for one device.
>>>>
>>>>
>>>> But if the particles are this expensive...
>>>>
>>>>
>>>>
>>>>
>>>>
>>>> On Mon, Aug 27, 2012 at 12:22 PM, Patrik D'haeseleer <pat...@gmail.com>wrote:
>>>>
>>>>> Here's some more useful DIY info:
>>>>>
>>>>> http://thalamus.wustl.edu/**nonetlab/ResourcesF/genegun/**Genegun.htm<http://thalamus.wustl.edu/nonetlab/ResourcesF/genegun/Genegun.htm>
>>>>> http://dictybase.org/**techniques/particle%20gun/**particle_gun.htm<http://dictybase.org/techniques/particle%20gun/particle_gun.htm>
>>>>> http://diagnosisp.com/dp/**journals/view_pdf.php?journal_**
>>>>> id=1&archive=0&issue_id=5&**article_id=148<http://diagnosisp.com/dp/journals/view_pdf.php?journal_id=1&archive=0&issue_id=5&article_id=148>
>>>>>
>>>>>
>>>>>
>>>>>
>>>>> On Monday, August 27, 2012 3:03:45 AM UTC-7, Patrik D'haeseleer wrote:
>>>>>>
>>>>>> We were tossing around the idea of a DIY gene gun at BioCurious as
>>>>>> well. Sure, helium and gold particles may be more efficient, but I bet a
>>>>>> hacked bb gun and, say, magnetite particles may give at least some
>>>>>> transformation as well.
>>>>>>
>>>>>> Here's a great video on how to prepare DNA "bullets", and how to use
>>>>>> the gene gun to transform a small selection of cells in live neuronal
>>>>>> samples:
>>>>>>
>>>>>> http://www.jove.com/index/**deta**ils.stp?id=675<http://www.jove.com/index/details.stp?id=675>
>>>>>>
>>>>>> Patrik
>>>>>>
>>>>>> On Sunday, August 26, 2012 3:15:53 AM UTC-7, Mega wrote:
>>>>>>>
>>>>>>> Hi,
>>>>>>>
>>>>>>> I 'm wondering how one could build such a device for particle
>>>>>>> bombardement.
>>>>>>>
>>>>>>> They usually use Helium, but why not Nitrogen or Carbon dioxide
>>>>>>> (widely available). I read they use low pressure, but what is low?
>>>>>>> Does it have to be in a vakuum??? I can do this, but it will be
>>>>>>> expensive and difficult to build the vakuum-tight casing and buy the vakuum
>>>>>>> pump.
>>>>>>>
>>>>>>> Can there be too much pressure (so the particles fly through the
>>>>>>> cells without leaving DNA)? Or can I configure it just using 'some
>>>>>>> pressure' ?
>>>>>>>
>>>>>>>
>>>>>>> (I have to look up the laws of my country, but as you cannot harm
>>>>>>> anyone with this device, it shouldn't be an issue)
>>>>>>>
>>>>>>>
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>>>>
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>
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--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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