Tungsten is "nearly" (very closely) as heavy as gold.
( I heard that criminals produce barren with >90% tungsten (in the core) and some gold around, and it gets confused with pure gold sometimes even by professionals )
Tungsten is way cheaper than gold. So it may be the way to go... Or is magnetite (iron is, definitely, but does it have the effects?) even more "much cheaper" than tungsten??
On Mon, Aug 27, 2012 at 8:35 PM, Patrik D'haeseleer <patrikd@gmail.com> wrote:
That's a great find!
I think we'll just have to experiment a bit to find the right particle composition. Obviously, you want something that is relatively inert, preferably high density, and very small particle size. So for a professional lab, gold or tungsten nanoparticles are an obvious choice. That does *not* mean this is the only way to get it to work though!
I brought up magnetite particles before, because I'd been looking into how to make ferrofluid lately. It turns out there's a simple chemical route to make magnetite nanoparticles that are way smaller than the magnetite powder I had bought online in initially. And magnetite may not be as heavy as gold, but I can tell you that jar of magnetite has some serious heft to it. Probably worth a try. Plus, the magnetic nature of the particles may allow us to manipulate them in ways you can't do with gold.
Patrik
On Monday, August 27, 2012 8:38:46 AM UTC-7, Mega wrote:I was thinking in investing into http://www.alibaba.com/product-gs/226547546/Portable_Gene_Gun.html this one. 300$ for one device.
But if the particles are this expensive...On Mon, Aug 27, 2012 at 12:22 PM, Patrik D'haeseleer <pat...@gmail.com> wrote:
Here's some more useful DIY info:
http://thalamus.wustl.edu/nonetlab/ResourcesF/genegun/Genegun.htm
http://dictybase.org/techniques/particle%20gun/particle_gun.htm
http://diagnosisp.com/dp/journals/view_pdf.php?journal_id=1&archive=0&issue_id=5&article_id=148To post to this group, send email to diy...@googlegroups.com.
On Monday, August 27, 2012 3:03:45 AM UTC-7, Patrik D'haeseleer wrote:We were tossing around the idea of a DIY gene gun at BioCurious as well. Sure, helium and gold particles may be more efficient, but I bet a hacked bb gun and, say, magnetite particles may give at least some transformation as well.--
Here's a great video on how to prepare DNA "bullets", and how to use the gene gun to transform a small selection of cells in live neuronal samples:
http://www.jove.com/index/details.stp?id=675
Patrik
On Sunday, August 26, 2012 3:15:53 AM UTC-7, Mega wrote:Hi,
I 'm wondering how one could build such a device for particle bombardement.
They usually use Helium, but why not Nitrogen or Carbon dioxide (widely available). I read they use low pressure, but what is low?
Does it have to be in a vakuum??? I can do this, but it will be expensive and difficult to build the vakuum-tight casing and buy the vakuum pump.
Can there be too much pressure (so the particles fly through the cells without leaving DNA)? Or can I configure it just using 'some pressure' ?
(I have to look up the laws of my country, but as you cannot harm anyone with this device, it shouldn't be an issue)
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