Re: [DIYbio] Re: DIY media for bioluminescente algae

Hi Loïc,

We've actually been doing a bunch of growth experiments with P. lunula at BioCurious lately. We handed out around 250 vials of dinoflagellates at the Bay Area Maker Faire a couple of months ago, along with a link to the following page of instructions:

http://tinyurl.com/MyPetDinos  (I'll be updating that one soon with our latest results)

The official growth medium (L1 or f/2) is mostly nitrate by weight, so we figured we would give plant fertilizers a try. Turns out they grow on pretty much anything we've tried, provided you get the concentration in the right neighborhood. Higher concentrations of plant fertilizer do tend to cause more problems with cyanobacterial contamination, presumably because of the higher phosphorus levels than are found in the official medium. We've been getting our salt water from a local aquarium store, but you may be able to avoid this problem by sterilizing the water, or mixing it yourself from filtered water plus sea salts.

What size of "massive" growing do you have in mind here? Plant fertilizers are great because you can tell any ten year old how to grow dinoflagellates. But if you're really planning to grow massive amounts in a more controlled environment, you may want to have a look at the "Micro Algae Grow Mass Pack" here:

http://florida-aqua-farms.com/secure/agora.cgi?cart_id=3307010.22134*Ua7qr2&product=MICRO_MACRO_NUTRIENTS

One kg of the dry nutrients only costs $20 plus shipping, and is enough to make 10,000 liters! At that level, it's going to cost you more to filter or sterilize that much salt water...

On Monday, August 27, 2012 2:16:11 AM UTC-7, Loïc wrote:

Hi everybody,
Does anybody knows a DIY liquid media for growing bioluminescence algae like  Pyrocystis lunula.
I grow this algae with a standard laboratory media, but it's too expensive for massive growing.
Thank you for yours answers.
.
Thanks

Best regards

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