This is pretty neat, if I remember you are the one that payed to get the Neurospora genome sequenced for your own data?
How did you go about chosing these 1014 bps for your synthetic promoter? I imagine there are multiple promoter sequences, what about an entirely new one?
I was just messing around looking at the H1 gene, but I get so lost in genome software and usually quit from frustration. I just want to see the ssDNA sequence upstream and downstream of that gene but can't figure out how.
Out of curiosity, why are those repeat homopolymers needed? The cytosine run of 11 bp's in the already rich GC area seems to be something they had trouble with.
Unfortunately I do not have experience with this to offer any help, but it is a pretty sweet project!
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