Re: [DIYbio] writing DNA

http://en.wikipedia.org/wiki/Directionality_(molecular_biology)

On Wed, Aug 29, 2012 at 3:24 PM, Mega <masterstorm123@gmail.com> wrote:
> Hallo guys,
>
> I'm desperately looking for some good websites which explain how DNA is
> written (by nature or by human).
>
>
> For example:
>
> [.....] that promoter activity of a 257 nucleotide fragment (-257 to the
> transcription initiation site) and a 241 nucleotide fragment (-241 to the
> transcription initiation site) were 5-fold and 2-fold stronger than that of
> the full-length CLCuMV C1 promoter respectively.
>
> (5') TCATGATTACGGGAGCGTAAAATACAAATTAAATCCCGTATTAACTCGTACCCAAGCGTA
> TCCGGTGAATAATCCTGCGACAAAAGTTGCAAATATTTCACAGAAAGCATACACCGAAAC
> CCGTGAACCGTATCAGGGAATTCGTTTAATAGTGGATCCCACATGTTTGAATTTGAAACT
> TAGTGCGCAAGTACTTATAGTGTGCGGGAGCGTTATTTAGCTTTGAGGGAGCAATCTCGT
> AAATCGGGGGCCCACAAAAAAAAAAGCGCGGCCATCCGGTAATATTATACGGATGGCCGC
> TTTTTGGAGCGTGAGGATTTTGAAATGATTTCTCAAATTACGATAATGCCATTTGGGGTA
> CACCTATATATTGCACCCCGTTACACCGATTGCCAGAGAATTAGAGTGTACACCGATTGC
> CACCATG (3')
>
>
> Is the red string the core promotor I'm looking for?? Or is it the
> underlined 257 bp fragment?? Where do you start counting (the TIS must be at
> the 3' end, I think?? )
>
>
>
> thx
>
>
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>



--
Nathan McCorkle
Rochester Institute of Technology
College of Science, Biotechnology/Bioinformatics

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