Does anyone by chance know how long an amino acid chain can be without getting problems with transcription and/or translation?
Say you want to make a 6kbp long fusion protein (you clone the genes in frame and remove the stop codons) . That would make 2000 amino acids(!), and those sequences I've seen so far have around 400 amino acids.
Will there be problems with the RNA polymerase or during translation?
(Google didn't hit any adequate results, unfortunately)
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