[DIYbio] Re: Now, about that gold gun...

I was also looking to see if Helium could be replaced with something more "portable" (like CO2 cartridges) and found this article.


"With the self-design CO2 propelled gene gun (200 
psi, distance 3 cm with 400 mesh nylon screen) using 
tungsten particle (600 nm diameter) coated with plasmid 
expressing anti-ampicillin gene, the plasmid with antiampicillin gene was high efficiently transferred into E. 


This link also has an interesting approach to prevent harm by preventing the shock wave of gas from directly hitting the culture. If you could get both of these to work a smaller portable gun should be possible.

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